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Literature summary for 2.6.1.57 extracted from

  • Beschle, H.G.; Süssmuth, R.; Lingens, F.
    Properties of aromatic-amino-acid aminotransferases from two chloramphenicol-resistant Flavobacteria (1982), Hoppe-Seyler's Z. Physiol. Chem., 363, 1365-1375.
    View publication on PubMed

General Stability

General Stability Organism
2-oxoglutarate or pyridoxal 5'-phosphate stabilizes against heat inactivation Flavobacterium sp.

Inhibitors

Inhibitors Comment Organism Structure
cycloserine 0.15 mM, 89% inhibition of aromatic aminotransferase from strain CB 6 Flavobacterium sp.
Isonicotinohydrazide 36% inhibition of aromatic aminotransferase from strain CB 60, enzyme from strain CB 6 is not inhibited Flavobacterium sp.
additional information enzyme from strain CB 60 is not inhibited by cycloserine Flavobacterium sp.
phenylhydrazine 0.15 mM, 78% inhibition of aromatic aminotransferase from strain CB 60, 56% inhibition of enzyme from strain CB 6 Flavobacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.97
-
2-oxoglutarate aromatic amino acid transferase from strain CB 6 Flavobacterium sp.
1.33
-
L-phenylalanine aromatic amino acid transferase from strain CB 6 Flavobacterium sp.
1.37
-
L-tryptophan aromatic amino acid transferase from strain CB 6 Flavobacterium sp.
1.4
-
L-tyrosine aromatic amino acid transferase from strain CB 6 Flavobacterium sp.
1.7
-
2-oxoglutarate aromatic amino acid transferase from strain CB 60 Flavobacterium sp.
15
-
L-tyrosine aromatic amino acid transferase from strain CB 60 Flavobacterium sp.
16.6
-
L-phenylalanine aromatic amino acid transferase from strain CB 60 Flavobacterium sp.
16.6
-
L-tryptophan aromatic amino acid transferase from strain CB 60 Flavobacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
enzymes from strain CB 60 and CB 6, gel filtration Flavobacterium sp.

Organism

Organism UniProt Comment Textmining
Flavobacterium sp.
-
strains CB 60 and CB 6
-

Purification (Commentary)

Purification (Comment) Organism
strain CB 60, ammonium sulfate, DEAE-cellulose, Ultrogel AcA 34, strain CB 6, DEAE-cellulose, Ultrogel AcA 34 Flavobacterium sp.

Reaction

Reaction Comment Organism Reaction ID
an aromatic amino acid + 2-oxoglutarate = an aromatic oxo acid + L-glutamate ping-pong bi-bi mechanism Flavobacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.49
-
strain CB 6 Flavobacterium sp.
1.59
-
strain CB 60 Flavobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-3-phenylpropanol + 2-oxoglutarate aromatic aminotransferase from strain CB 6 Flavobacterium sp. 2-oxo-3-phenylpropanol + L-glutamate
-
?
L-phenylalanine + 2-oxoglutarate
-
Flavobacterium sp. phenylpyruvate + L-glutamate
-
?
L-phenylalanine methylester + 2-oxoglutarate aromatic aminotransferase from strain CB 6 Flavobacterium sp. 2-oxo-3-phenylpropanoate methylester + L-glutamate
-
?
L-tryptophan + 2-oxoglutarate
-
Flavobacterium sp. 3-indole-2-oxopropanoate + L-glutamate
-
?
L-tyrosine + 2-oxoglutarate
-
Flavobacterium sp. 4-hydroxyphenylpyruvate + L-glutamate
-
?
p-aminophenylalanine + 2-oxoglutarate aromatic aminotransferase from strain CB 60 and CB 6 Flavobacterium sp. p-aminophenylpyruvate + L-glutamate
-
?
p-aminophenylserine + 2-oxoglutarate aromatic aminotransferase from strain CB 60 and CB 6 Flavobacterium sp. 3-hydroxy-2-oxo-3-(p-aminophenyl)propanoic acid + L-glutamate
-
?
p-aminophenylserinol + 2-oxoglutarate aromatic aminotransferase from strain CB 60 and CB 6 Flavobacterium sp. 3-(p-aminophenyl)-1,3-propanediol + L-glutamate
-
?
p-nitrophenylserine + 2-oxoglutarate aromatic aminotransferase from strain CB 60 and CB 6 Flavobacterium sp. 3-hydroxy-2-oxo-3-(p-nitrophenyl)propanoic acid + L-glutamate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
strain CB 60 Flavobacterium sp.
57.5
-
strain CB 6 Flavobacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
2-oxoglutarate or pyridoxal 5'-phosphate stabilize against heat inactivation Flavobacterium sp.
55
-
strain CB 60, complete inactivation after 8 min, 28% and 8% activity remain after 8 min in the presence of 2-oxoglutarate, strain CB 6, 68% loss of activity after 32 min, 35% and 22% loss of activity after 32 min in the presence of pyridoxal 5'-phosphate and 2-oxoglutarate respectively Flavobacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 9 strain CB 6 Flavobacterium sp.
8.4 9 strain CB 60 Flavobacterium sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 10 approx. 50% of maximal activity at pH 6.0 Flavobacterium sp.
6 10 approx. 60% of maximal activity at pH 10.0 Flavobacterium sp.
7 9.5 approx. 30% of maximal activity at pH 7.0 and pH 9.5 Flavobacterium sp.

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate tightly bound to enzyme Flavobacterium sp.