General Stability | Organism |
---|---|
2-oxoglutarate or pyridoxal 5'-phosphate stabilizes against heat inactivation | Flavobacterium sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cycloserine | 0.15 mM, 89% inhibition of aromatic aminotransferase from strain CB 6 | Flavobacterium sp. | |
Isonicotinohydrazide | 36% inhibition of aromatic aminotransferase from strain CB 60, enzyme from strain CB 6 is not inhibited | Flavobacterium sp. | |
additional information | enzyme from strain CB 60 is not inhibited by cycloserine | Flavobacterium sp. | |
phenylhydrazine | 0.15 mM, 78% inhibition of aromatic aminotransferase from strain CB 60, 56% inhibition of enzyme from strain CB 6 | Flavobacterium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.97 | - |
2-oxoglutarate | aromatic amino acid transferase from strain CB 6 | Flavobacterium sp. | |
1.33 | - |
L-phenylalanine | aromatic amino acid transferase from strain CB 6 | Flavobacterium sp. | |
1.37 | - |
L-tryptophan | aromatic amino acid transferase from strain CB 6 | Flavobacterium sp. | |
1.4 | - |
L-tyrosine | aromatic amino acid transferase from strain CB 6 | Flavobacterium sp. | |
1.7 | - |
2-oxoglutarate | aromatic amino acid transferase from strain CB 60 | Flavobacterium sp. | |
15 | - |
L-tyrosine | aromatic amino acid transferase from strain CB 60 | Flavobacterium sp. | |
16.6 | - |
L-phenylalanine | aromatic amino acid transferase from strain CB 60 | Flavobacterium sp. | |
16.6 | - |
L-tryptophan | aromatic amino acid transferase from strain CB 60 | Flavobacterium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
enzymes from strain CB 60 and CB 6, gel filtration | Flavobacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Flavobacterium sp. | - |
strains CB 60 and CB 6 | - |
Purification (Comment) | Organism |
---|---|
strain CB 60, ammonium sulfate, DEAE-cellulose, Ultrogel AcA 34, strain CB 6, DEAE-cellulose, Ultrogel AcA 34 | Flavobacterium sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an aromatic amino acid + 2-oxoglutarate = an aromatic oxo acid + L-glutamate | ping-pong bi-bi mechanism | Flavobacterium sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.49 | - |
strain CB 6 | Flavobacterium sp. |
1.59 | - |
strain CB 60 | Flavobacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-3-phenylpropanol + 2-oxoglutarate | aromatic aminotransferase from strain CB 6 | Flavobacterium sp. | 2-oxo-3-phenylpropanol + L-glutamate | - |
? | |
L-phenylalanine + 2-oxoglutarate | - |
Flavobacterium sp. | phenylpyruvate + L-glutamate | - |
? | |
L-phenylalanine methylester + 2-oxoglutarate | aromatic aminotransferase from strain CB 6 | Flavobacterium sp. | 2-oxo-3-phenylpropanoate methylester + L-glutamate | - |
? | |
L-tryptophan + 2-oxoglutarate | - |
Flavobacterium sp. | 3-indole-2-oxopropanoate + L-glutamate | - |
? | |
L-tyrosine + 2-oxoglutarate | - |
Flavobacterium sp. | 4-hydroxyphenylpyruvate + L-glutamate | - |
? | |
p-aminophenylalanine + 2-oxoglutarate | aromatic aminotransferase from strain CB 60 and CB 6 | Flavobacterium sp. | p-aminophenylpyruvate + L-glutamate | - |
? | |
p-aminophenylserine + 2-oxoglutarate | aromatic aminotransferase from strain CB 60 and CB 6 | Flavobacterium sp. | 3-hydroxy-2-oxo-3-(p-aminophenyl)propanoic acid + L-glutamate | - |
? | |
p-aminophenylserinol + 2-oxoglutarate | aromatic aminotransferase from strain CB 60 and CB 6 | Flavobacterium sp. | 3-(p-aminophenyl)-1,3-propanediol + L-glutamate | - |
? | |
p-nitrophenylserine + 2-oxoglutarate | aromatic aminotransferase from strain CB 60 and CB 6 | Flavobacterium sp. | 3-hydroxy-2-oxo-3-(p-nitrophenyl)propanoic acid + L-glutamate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
strain CB 60 | Flavobacterium sp. |
57.5 | - |
strain CB 6 | Flavobacterium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
2-oxoglutarate or pyridoxal 5'-phosphate stabilize against heat inactivation | Flavobacterium sp. |
55 | - |
strain CB 60, complete inactivation after 8 min, 28% and 8% activity remain after 8 min in the presence of 2-oxoglutarate, strain CB 6, 68% loss of activity after 32 min, 35% and 22% loss of activity after 32 min in the presence of pyridoxal 5'-phosphate and 2-oxoglutarate respectively | Flavobacterium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 9 | strain CB 6 | Flavobacterium sp. |
8.4 | 9 | strain CB 60 | Flavobacterium sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | approx. 50% of maximal activity at pH 6.0 | Flavobacterium sp. |
6 | 10 | approx. 60% of maximal activity at pH 10.0 | Flavobacterium sp. |
7 | 9.5 | approx. 30% of maximal activity at pH 7.0 and pH 9.5 | Flavobacterium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | tightly bound to enzyme | Flavobacterium sp. |