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Literature summary for 2.6.1.57 extracted from

  • Lee, C.W.; Desmazeaud, M.J.
    Partial purification and some properties of an aromatic-amino-acid and an aspartate aminotransferase in Brevibacterium linens 47 (1985), J. Gen. Microbiol., 131, 459-467.
No PubMed abstract available
Search for more literature for 2.6.1.57

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
 L-tryptophan pH 8.5, 37°C Brevibacterium linens
1.3
-
 L-phenylalanine pH 8.5, 37°C Brevibacterium linens
1.4
-
 L-tyrosine pH 8.5, 37°C Brevibacterium linens
8.4
-
 L-aspartate pH 8.5, 37°C Brevibacterium linens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
126000
-
 gel filtration Brevibacterium linens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-phenylalanine + 2-oxoglutarate Brevibacterium linens probably key enzyme for utilization of aromatic amino acids as sole nitrogen source in Brevibacterium linens 47 phenylpyruvate + L-glutamate
-
 ?
L-phenylalanine + 2-oxoglutarate Brevibacterium linens 47 probably key enzyme for utilization of aromatic amino acids as sole nitrogen source in Brevibacterium linens 47 phenylpyruvate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Brevibacterium linens
-
-
-
Brevibacterium linens 47
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Streptomycin sulfate and ammonium sulfate, DEAE-Trisacryl, HA-ultrogel, Sephacryl S-200, partial purification Brevibacterium linens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8
-
 with tyrosine Brevibacterium linens
2.96
-
 with aspartate Brevibacterium linens
3.8
-
 with tryptophan Brevibacterium linens
6.5
-
 with phenylalanine Brevibacterium linens

Storage Stability

Storage Stability Organism
4°C, 100 mM potassium phosphate buffer, pH 7.5, 0.002% NaN3, at least 6 months, no loss of activity Brevibacterium linens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aspartate + 2-oxoglutarate
-
 Brevibacterium linens oxaloacetate + L-glutamate
-
 ?
L-methionine + 2-oxoglutarate
-
 Brevibacterium linens 4-methylsulfanyl-2-oxobutanoate + L-glutamate
-
 ?
L-phenylalanine + 2-oxoglutarate completely inactive with D-isomers of aromatic amino acids Brevibacterium linens phenylpyruvate + L-glutamate
-
 ?
L-phenylalanine + 2-oxoglutarate probably key enzyme for utilization of aromatic amino acids as sole nitrogen source in Brevibacterium linens 47 Brevibacterium linens phenylpyruvate + L-glutamate
-
 ?
L-phenylalanine + 2-oxoglutarate completely inactive with D-isomers of aromatic amino acids Brevibacterium linens 47 phenylpyruvate + L-glutamate
-
 ?
L-phenylalanine + 2-oxoglutarate probably key enzyme for utilization of aromatic amino acids as sole nitrogen source in Brevibacterium linens 47 Brevibacterium linens 47 phenylpyruvate + L-glutamate
-
 ?
L-phenylalanine + oxaloacetate
-
 Brevibacterium linens phenylpyruvate + L-aspartate
-
 ?
L-phenylalanine + oxaloacetate
-
 Brevibacterium linens 47 phenylpyruvate + L-aspartate
-
 ?
L-tryptophan + 2-oxoglutarate
-
 Brevibacterium linens 3-indole-2-oxopropanoate + L-glutamate
-
 ?
L-tryptophan + 2-oxoglutarate
-
 Brevibacterium linens 47 3-indole-2-oxopropanoate + L-glutamate
-
 ?
L-tryptophan + oxaloacetate
-
 Brevibacterium linens 3-indole-2-oxopropanoate + L-aspartate
-
 ?
L-tyrosine + 2-oxoglutarate
-
 Brevibacterium linens p-hydroxyphenylpyruvate + L-glutamate
-
 ?
L-tyrosine + 2-oxoglutarate
-
 Brevibacterium linens 47 p-hydroxyphenylpyruvate + L-glutamate
-
 ?

Synonyms

Synonyms Comment Organism
AT-IA
-
 Brevibacterium linens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37 40 at pH 8.5 Brevibacterium linens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9 phenylalanine Brevibacterium linens

pH Range

pH Minimum pH Maximum Comment Organism
6.5 10 approx. 45% of maximal activity at pH 6.5, approx. 50% of maximal activity at pH 10 Brevibacterium linens

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate a pyridoxal phosphate protein Brevibacterium linens
pyridoxal 5'-phosphate tightly bound to enzyme Brevibacterium linens