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Literature summary for 2.6.1.54 extracted from

  • Tani, Y.; Ukita, M.; Ogata, K.
    Studies on vitamin b6 metabolism in microorganisms. Part X. Further purification and characterization of pyridoxamine 5'-phosphate-alpha-ketoglutarate transaminase from Clostridium kainantoi (1972), Agric. Biol. Chem., 36, 181-188.
No PubMed abstract available
Search for more literature for 2.6.1.54

Activating Compound

Activating Compound Comment Organism Structure
4-aminobutyrate stimulation, 177% of non-stimulated activity Clostridium butyricum
D-asparagine stimulation, 112% of non-stimulated activity Clostridium butyricum
D-cysteine stimulation, 135% of non-stimulated activity Clostridium butyricum
D-methionine stimulation, 127% of non-stimulated activity Clostridium butyricum
DL-Ornithine stimulation, 112% of non-stimulated activity Clostridium butyricum
glycine stimulation, 137% of non-stimulated activity Clostridium butyricum
L-asparagine stimulation, 112% of non-stimulated activity Clostridium butyricum
L-glutamate stimulation, 166% of non-stimulated activity Clostridium butyricum
L-lysine stimulation, 105% of non-stimulated activity Clostridium butyricum
L-methionine stimulation, 114% of non-stimulated activity Clostridium butyricum
L-ornithine stimulation, 112% of non-stimulated activity Clostridium butyricum
additional information stimulating effect of amino compounds may be caused by disappearance of the inhibitory product of the reaction, pyridoxal 5'-phosphate, forming the corresponding Schiff base Clostridium butyricum

Inhibitors

Inhibitors Comment Organism Structure
D-glutamate slight inhibition Clostridium butyricum
diisopropyl phosphofluoride 0.3 mM, complete inhibition Clostridium butyricum
L-cysteine about 35% inhibition Clostridium butyricum
additional information not inhibited by sulfhydryl compounds, e.g. PCMB, phenylmercuric acetate and mercuric chloride Clostridium butyricum
pyridoxal 5'-phosphate product inhibition Clostridium butyricum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 pyridoxamine 5'-phosphate pH 8.0, 37°C Clostridium butyricum
0.01
-
 pyridoxal 5'-phosphate pH 8.0, 37°C Clostridium butyricum
0.015
-
 2-oxoglutarate cosubstrate pyridoxamine 5'-phosphate, pH 8.0, 37°C Clostridium butyricum
9
-
 D-glutamate pH 8.0, 37°C Clostridium butyricum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information metal ions do not accelerate the reaction Clostridium butyricum

Organism

Organism UniProt Comment Textmining
Clostridium butyricum
-
 IFO 3353
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, protamine treatment, DEAE-cellulose, hydroxylapatite, DEAE-Sephadex, Sephadex G-200, partial purification Clostridium butyricum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.281
-
-
 Clostridium butyricum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-aminobutanoate + 2-oxoglutarate about 8% of activity compared to pyridoxamine 5'-phosphate Clostridium butyricum 4-oxobutanoate + D-glutamate
-
 ?
pyridoxamine + 2-oxoglutarate about 25% of activity compared to pyridoxamine 5'-phosphate Clostridium butyricum pyridoxal + D-glutamate
-
 ?
pyridoxamine 5'-phosphate + 2-oxoglutarate
-
 Clostridium butyricum pyridoxal 5'-phosphate + D-glutamate
-
 r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Clostridium butyricum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Clostridium butyricum