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Literature summary for 2.6.1.52 extracted from

  • Koivulehto, M.; Battchikova, N.; Korpela, S.; Khalikova, E.; Zavialov, A.; Korpela, T.
    Comparison of kinetic and enzymatic properties of intracellular phosphoserine aminotransferases from alkaliphilic and neutralophilic bacteria (2020), Open Chem., 18, 149-164 .
No PubMed abstract available
Search for more literature for 2.6.1.52

Cloned(Commentary)

Cloned (Comment) Organism
gene serC, recombinant overexpression in Escherichia coli strain AB2829 harbouring pKD501 plasmid Escherichia coli
gene serC, recombinant overexpression in Escherichia coli strain BL21(DE3) Alkalihalobacillus alcalophilus
gene serC, recombinant overexpression of enzyme mutant K2G in Escherichia coli strain XL-1 Blue Niallia circulans

Protein Variants

Protein Variants Comment Organism
K2G site-directed mutagenesis Niallia circulans
additional information mutant C2-A' Tetradesmus obliquus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information enzyme kinetics, overview Niallia circulans
additional information
-
 additional information enzyme kinetics, overview Alkalihalobacillus alcalophilus
additional information
-
 additional information enzyme kinetics, overview Escherichia coli
0.0017
-
 3-phosphooxypyruvate pH 8.2, 25°C, recombinant enzyme mutant K2G Niallia circulans
0.004
-
 3-phosphooxypyruvate pH 8.2, 25°C, recombinant enzyme Escherichia coli
0.005
-
 3-phosphooxypyruvate pH 8.2, 25°C, recombinant enzyme Bos taurus
0.0173
-
 3-phosphooxypyruvate pH 9.5, 25°C, recombinant enzyme mutant K2G Niallia circulans
0.06
-
 3-phosphooxypyruvate pH 7.5-9.0, 25°C, recombinant enzyme Glycine max
0.25
-
 3-phosphooxypyruvate pH 8.2, 25°C, recombinant enzyme Ovis aries
0.5
-
 L-glutamate pH 7.5-9.0, 25°C, recombinant enzyme Glycine max
0.8
-
 L-glutamate pH 9.5, 25°C, recombinant enzyme mutant K2G Niallia circulans
1.2
-
 L-glutamate pH 8.2, 25°C, recombinant enzyme Escherichia coli
1.2
-
 L-glutamate pH 8.2, 25°C, recombinant enzyme Bos taurus
1.4
-
 L-glutamate pH 8.2, 25°C, recombinant enzyme mutant K2G Niallia circulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 recombinant enzyme K2G, gel filtration Niallia circulans
68000
-
 gel filtration Escherichia coli
77000
-
 gel filtration Bos taurus
79800 81200
-
 Escherichia coli
80000
-
 gel filtration Tetradesmus obliquus
85000
-
 gel filtration Glycine max
90700
-
 sedimentation equilibrium Bos taurus
96000
-
 sedimentation equilibrium Ovis aries

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
O-phospho-L-serine + 2-oxoglutarate Niallia circulans
-
 3-phosphooxypyruvate + L-glutamate
-
 r
O-phospho-L-serine + 2-oxoglutarate Alkalihalobacillus alcalophilus
-
 3-phosphooxypyruvate + L-glutamate
-
 r
O-phospho-L-serine + 2-oxoglutarate Escherichia coli
-
 3-phosphooxypyruvate + L-glutamate
-
 r
O-phospho-L-serine + 2-oxoglutarate Bos taurus
-
 3-phosphooxypyruvate + L-glutamate
-
 r
O-phospho-L-serine + 2-oxoglutarate Ovis aries
-
 3-phosphooxypyruvate + L-glutamate
-
 r
O-phospho-L-serine + 2-oxoglutarate Tetradesmus obliquus
-
 3-phosphooxypyruvate + L-glutamate
-
 r
O-phospho-L-serine + 2-oxoglutarate Glycine max
-
 3-phosphooxypyruvate + L-glutamate
-
 r

Organism

Organism UniProt Comment Textmining
Alkalihalobacillus alcalophilus Q9RME2 i.e. Bacillus alcalophilus
-
Bos taurus A0A3S5ZPF7
-
-
Escherichia coli E2QJB8
-
-
Escherichia coli P23721
-
-
Glycine max I1JCA9
-
-
Niallia circulans Q59196 var. alcalophilus
-
Ovis aries W5PS11
-
-
Tetradesmus obliquus Q9S8N7 i.e. Acutodesmus obliquus
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme 15.6fold from Escherichia coli strain BL21(DE3) by two different steps of anion exchange chromatography, ultrafiltration, and gel filtration Alkalihalobacillus alcalophilus
recombinant enzyme mutant K2G 5.5fold from Escherichia coli strain XL-1 Blue by anion exchange chromatography, ultrafiltration, and gel filtration Niallia circulans

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Ovis aries
-
liver
-
 Bos taurus
-
additional information the alkaliphilic microbe grows optimally at pH 9.5-10.5 Niallia circulans
-
root nodule
-
 Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3
-
 purified enzyme, pH 8.1-8.2, 25°C Ovis aries
3.8
-
 pH not specified in the publication, 25°C, purified recombinant enzyme, substrate 3-phosphooxypyruvate Escherichia coli
7
-
 purified enzyme, pH and temperature not specified in the publication Glycine max
42
-
 purified recombinant enzyme, pH 6.1, 25°C Alkalihalobacillus alcalophilus
42.3
-
 purified recombinant enzyme, pH 8.2, 25°C Escherichia coli
44.4
-
 purified recombinant enzyme mutant K2G, pH 6.1, 25°C Niallia circulans
1416
-
 purified enzyme, pH 6.8-7.2, 38°C Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-phosphooxypyruvate + L-glutamate
-
 Niallia circulans O-phospho-L-serine + 2-oxoglutarate
-
 r
3-phosphooxypyruvate + L-glutamate
-
 Alkalihalobacillus alcalophilus O-phospho-L-serine + 2-oxoglutarate
-
 r
3-phosphooxypyruvate + L-glutamate
-
 Escherichia coli O-phospho-L-serine + 2-oxoglutarate
-
 r
3-phosphooxypyruvate + L-glutamate
-
 Bos taurus O-phospho-L-serine + 2-oxoglutarate
-
 r
3-phosphooxypyruvate + L-glutamate
-
 Ovis aries O-phospho-L-serine + 2-oxoglutarate
-
 r
3-phosphooxypyruvate + L-glutamate
-
 Tetradesmus obliquus O-phospho-L-serine + 2-oxoglutarate
-
 r
3-phosphooxypyruvate + L-glutamate
-
 Glycine max O-phospho-L-serine + 2-oxoglutarate
-
 r
O-phospho-L-serine + 2-oxoglutarate
-
 Niallia circulans 3-phosphooxypyruvate + L-glutamate
-
 r
O-phospho-L-serine + 2-oxoglutarate
-
 Alkalihalobacillus alcalophilus 3-phosphooxypyruvate + L-glutamate
-
 r
O-phospho-L-serine + 2-oxoglutarate
-
 Escherichia coli 3-phosphooxypyruvate + L-glutamate
-
 r
O-phospho-L-serine + 2-oxoglutarate
-
 Bos taurus 3-phosphooxypyruvate + L-glutamate
-
 r
O-phospho-L-serine + 2-oxoglutarate
-
 Ovis aries 3-phosphooxypyruvate + L-glutamate
-
 r
O-phospho-L-serine + 2-oxoglutarate
-
 Tetradesmus obliquus 3-phosphooxypyruvate + L-glutamate
-
 r
O-phospho-L-serine + 2-oxoglutarate
-
 Glycine max 3-phosphooxypyruvate + L-glutamate
-
 r

Subunits

Subunits Comment Organism
? x * 39500-40500, recombinant enzyme, SDS-PAGE, x * 40200, about, sequence calculation Alkalihalobacillus alcalophilus
homodimer 2 * 40000, SDS-PAGE Tetradesmus obliquus
homodimer 2 * 43000, SDS-PAGE Bos taurus
homodimer 2 * 38000-39800, recombinant enzyme mutant K2G, SDS-PAGE, 2 * 39793, sequence calculation Niallia circulans
homodimer 2 * 39000-40000, SDS-PAGE, 2 * 39834, sequence calculation Escherichia coli

Synonyms

Synonyms Comment Organism
phosphoserine aminotransferases
-
 Niallia circulans
phosphoserine aminotransferases
-
 Alkalihalobacillus alcalophilus
phosphoserine aminotransferases
-
 Escherichia coli
phosphoserine aminotransferases
-
 Bos taurus
phosphoserine aminotransferases
-
 Ovis aries
phosphoserine aminotransferases
-
 Tetradesmus obliquus
phosphoserine aminotransferases
-
 Glycine max
PSAT
-
 Niallia circulans
PSAT
-
 Alkalihalobacillus alcalophilus
PSAT
-
 Escherichia coli
PSAT
-
 Bos taurus
PSAT
-
 Ovis aries
PSAT
-
 Tetradesmus obliquus
PSAT
-
 Glycine max
PSAT-BALC
-
 Alkalihalobacillus alcalophilus
PSAT-BCIRA
-
 Niallia circulans
PSAT-ECOLI
-
 Escherichia coli
PSAT1
-
 Bos taurus
serC
-
 Niallia circulans
serC
-
 Alkalihalobacillus alcalophilus
serC
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Niallia circulans
25
-
 assay at Alkalihalobacillus alcalophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 7.2
-
 Bos taurus
6.8 8.2
-
 Tetradesmus obliquus
7.5 9 broad optimum Glycine max
7.5 8.5
-
 Escherichia coli
8.1 8.2
-
 Ovis aries
9.4
-
-
 Alkalihalobacillus alcalophilus
9.5
-
-
 Niallia circulans

pH Range

pH Minimum pH Maximum Comment Organism
6.5 12 activity range, profile overview Niallia circulans
6.5 12 activity range, profile overview Alkalihalobacillus alcalophilus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate PLP, dependent on Niallia circulans
pyridoxal 5'-phosphate PLP, dependent on Alkalihalobacillus alcalophilus
pyridoxal 5'-phosphate PLP, dependent on Escherichia coli
pyridoxal 5'-phosphate PLP, dependent on Bos taurus
pyridoxal 5'-phosphate PLP, dependent on Ovis aries
pyridoxal 5'-phosphate PLP, dependent on Tetradesmus obliquus
pyridoxal 5'-phosphate PLP, dependent on Glycine max

pI Value

Organism Comment pI Value Maximum pI Value
Niallia circulans isoelectric focusing
-
 4.6
Alkalihalobacillus alcalophilus isoelectric focusing
-
 4.6
Escherichia coli isoelectric focusing
-
 4.6

General Information

General Information Comment Organism
metabolism enzyme PSAT catalyses the second step of phosphorylated pathway of serine biosynthesis Niallia circulans
metabolism enzyme PSAT catalyses the second step of phosphorylated pathway of serine biosynthesis Alkalihalobacillus alcalophilus
metabolism enzyme PSAT catalyses the second step of phosphorylated pathway of serine biosynthesis Escherichia coli
metabolism enzyme PSAT catalyses the second step of phosphorylated pathway of serine biosynthesis Bos taurus
metabolism enzyme PSAT catalyses the second step of phosphorylated pathway of serine biosynthesis Ovis aries
metabolism enzyme PSAT catalyses the second step of phosphorylated pathway of serine biosynthesis Tetradesmus obliquus
metabolism enzyme PSAT catalyses the second step of phosphorylated pathway of serine biosynthesis Glycine max