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Literature summary for 2.6.1.52 extracted from

  • Haque, M.R.; Hirowatari, A.; Koyanagi, A.; Ichinose, T.; Abiru, M.; Mohri, S.; Furuya, S.; Yamamoto, K.
    Molecular characterization and expression analysis of a phosphoserine aminotransferase involving L-serine synthesis from silkworm, Bombyx mori (2019), Arch. Insect Biochem. Physiol., 101, e21553 .
    View publication on PubMed
Search for more literature for 2.6.1.52

Cloned(Commentary)

Cloned (Comment) Organism
sequence comparisons and phylogenetic analysis, quantitative PCR enzyme expression analysis, recombinant expression of SUMO-His6-tagged enzyme in Escherichia coli by using a cold-shock promotor Bombyx mori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.88
-
 L-glutamate pH 8.0, 25°C, recombinant enzyme Bombyx mori
6
-
 3-phosphooxypyruvate pH 8.0, 25°C, recombinant enzyme Bombyx mori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-phosphooxypyruvate + L-glutamate Bombyx mori
-
 O-phospho-L-serine + 2-oxoglutarate
-
 r
3-phosphooxypyruvate + L-glutamate Bombyx mori p50T
-
 O-phospho-L-serine + 2-oxoglutarate
-
 r
4-phosphooxy-L-threonine + 2-oxoglutarate Bombyx mori
-
 (3R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + L-glutamate
-
 r
4-phosphooxy-L-threonine + 2-oxoglutarate Bombyx mori p50T
-
 (3R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + L-glutamate
-
 r
O-phospho-L-serine + 2-oxoglutarate Bombyx mori
-
 3-phosphooxypyruvate + L-glutamate
-
 r
O-phospho-L-serine + 2-oxoglutarate Bombyx mori b94
-
 3-phosphooxypyruvate + L-glutamate
-
 r

Organism

Organism UniProt Comment Textmining
Bombyx mori H9JSH4
-
-
Bombyx mori Q2F5M8
-
-
Bombyx mori b94 Q2F5M8
-
-
Bombyx mori p50T H9JSH4
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant SUMO-His6-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration, to homogeneity Bombyx mori

Source Tissue

Source Tissue Comment Organism Textmining
fat body high expression level Bombyx mori
-
hemocyte low expression level Bombyx mori
-
larva
-
 Bombyx mori
-
midgut high expression level Bombyx mori
-
additional information the enzyme activity in strain p50T is higher compared to strain b94 Bombyx mori
-
ovary moderate expression level Bombyx mori
-
silk gland middle silk gland, high expression level Bombyx mori
-
testis moderate expression level Bombyx mori
-

Storage Stability

Storage Stability Organism
4°C, purified recombinant enzyme, pH 2.0-12.0, 24 h, over 80% activity remaining Bombyx mori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-phosphooxypyruvate + L-glutamate
-
 Bombyx mori O-phospho-L-serine + 2-oxoglutarate
-
 r
3-phosphooxypyruvate + L-glutamate
-
 Bombyx mori p50T O-phospho-L-serine + 2-oxoglutarate
-
 r
4-phosphooxy-L-threonine + 2-oxoglutarate
-
 Bombyx mori (3R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + L-glutamate
-
 r
4-phosphooxy-L-threonine + 2-oxoglutarate
-
 Bombyx mori p50T (3R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + L-glutamate
-
 r
additional information the enzyme preferentially utilizes glutamate for transamination Bombyx mori ?
-
-
additional information the enzyme preferentially utilizes glutamate for transamination Bombyx mori p50T ?
-
-
additional information the enzyme preferentially utilizes glutamate for transamination Bombyx mori b94 ?
-
-
O-phospho-L-serine + 2-oxoglutarate
-
 Bombyx mori 3-phosphooxypyruvate + L-glutamate
-
 r
O-phospho-L-serine + 2-oxoglutarate
-
 Bombyx mori b94 3-phosphooxypyruvate + L-glutamate
-
 r

Subunits

Subunits Comment Organism
? x * 40199, sequence calculation, x * 66000, recombinant SUMO-His6-tagged enzyme, SDS-PAGE Bombyx mori

Synonyms

Synonyms Comment Organism
bmPSAT
-
 Bombyx mori
phosphoserine aminotransferase
-
 Bombyx mori
PSAT
-
 Bombyx mori

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Bombyx mori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Bombyx mori

pH Range

pH Minimum pH Maximum Comment Organism
2 12 80% of maximal activity within this range Bombyx mori
4 10 50% of maximal activity within this range Bombyx mori

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 12 4°C, purified recombinant enzyme, 24 h, over 80% activity remaining Bombyx mori

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate PLP Bombyx mori

pI Value

Organism Comment pI Value Maximum pI Value
Bombyx mori sequence calculation
-
 6.97

General Information

General Information Comment Organism
malfunction a sericin-deficient silkworm strain exhibits a diminished expression of bmPSAT mRNA in the silk gland Bombyx mori
metabolism phosphoserine aminotransferase (bmPSAT) from Bombyx mori is responsible for L-serine biosynthesis. This pathway composed of D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), PSAT, and phosphoserine phosphatase (PSP, EC 3.1.3.3) is crucial for the de novo production of L-serine from a glycolytic intermediate, 3-phosphoglycerate Bombyx mori
physiological function phosphoserine aminotransferase (bmPSAT) from Bombyx mori is responsible for L-serine biosynthesis. bmPSAT may play an important role in synthesizing and supplying L-serine in the larva of Bombyx mori. The silk gland is the sole organ in which silk is synthesized and secreted in the silkworm. Silk is composed of two types of proteins: sericin and fibroin, both of which contain high levels of L-serine and glycine Bombyx mori