General Stability | Organism |
---|---|
enzyme can be inactivated by dialysis against cysteine | Ovis aries |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
3-phosphohydroxypyruvate | pH 8.2, 25°C | Ovis aries | |
0.7 | - |
L-glutamate | pH 8.2, 25°C | Ovis aries |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
96000 | - |
Trautman modification of the Archibald approach to equilibrium method | Ovis aries |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + 3-phosphohydroxypyruvate | Ovis aries | phosphorylated pathway for serine biosynthesis | O-phospho-L-serine + 2-oxoglutarate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ovis aries | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Ovis aries |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Ovis aries | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
13 U/mg | Ovis aries |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + 3-phosphonooxypyruvate | L-alanine as amino group donor, 10% of the activity with L-glutamate | Ovis aries | O-phospho-L-serine + pyruvate | - |
r | |
L-glutamate + 3-phosphohydroxypyruvate | forward reaction | Ovis aries | O-phospho-L-serine + 2-oxoglutarate | - |
r | |
L-glutamate + 3-phosphohydroxypyruvate | phosphorylated pathway for serine biosynthesis | Ovis aries | O-phospho-L-serine + 2-oxoglutarate | - |
r | |
additional information | hydroxypyruvate is unreactive | Ovis aries | ? | - |
? | |
O-phospho-L-serine + 2-oxoglutarate | reverse reaction | Ovis aries | 3-phosphonooxypyruvate + L-glutamate | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.15 | - |
- |
Ovis aries |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8.8 | at pH 7.8 about 45% of maximum activity, at pH 8.8 about 35% of maximum activity | Ovis aries |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Ovis aries |