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Literature summary for 2.6.1.51 extracted from

  • Oda, T.; Miyajima, H.; Suzuki, Y.; Ito, T.; Yokota, S.; Hoshino, M.; Ichiyama, A.
    Purification and characterization of the active serine: pyruvate aminotransferase of rat liver mitochondria expressed in Escherichia coli (1989), J. Biochem., 106, 460-467.
    View publication on PubMed
Search for more literature for 2.6.1.51

Cloned(Commentary)

Cloned (Comment) Organism
cDNA cloned and expressed in Escherichia coli DH1 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0246
-
 glyoxylate pH 8.0, 30°C, native enzyme Rattus norvegicus
0.0336
-
 glyoxylate pH 8.0, 30°C, recombinant enzyme Rattus norvegicus
1.1
-
 pyruvate pH 8.0, 30°C, native and recombinant enzyme Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Rattus norvegicus 5737
-
mitochondrion
-
 Rattus norvegicus 5739
-
peroxisome
-
 Rattus norvegicus 5777
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 2 * 43000, recombinant enzyme, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-serine + pyruvate Rattus norvegicus
-
 3-hydroxypyruvate + L-alanine
-
 r

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native and recombinant enzyme Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.41
-
 recombinant enzyme Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine + 3-hydroxypyruvate
-
 Rattus norvegicus pyruvate + L-serine
-
 r
L-serine + pyruvate
-
 Rattus norvegicus 3-hydroxypyruvate + L-alanine
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 43000, recombinant enzyme, SDS-PAGE Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Rattus norvegicus