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Literature summary for 2.6.1.5 extracted from

  • Sivaraman, S.; Kirsch, J.F.
    The narrow substrate specificity of human tyrosine aminotransferase--the enzyme deficient in tyrosinemia type II (2006), FEBS J., 273, 1920-1929.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
I249A reduced catalytic activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
67
-
L-tyrosine mutant enzyme I249A Homo sapiens
580
-
p-hydroxyphenylpyruvate wild type enzyme Homo sapiens
870
-
L-tyrosine wild type enzyme Homo sapiens
3300
-
2-oxoglutarate wild type enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
NTA-resin column chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + 4-hydroxyphenylpyruvate
-
Homo sapiens 2-oxoglutarate + L-tyrosine
-
?
L-tyrosine + 2-oxoglutarate
-
Homo sapiens 4-hydroxyphenylpyruvate + L-glutamate
-
?
additional information no detectable transamination of aspartate or other cosubstrates Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
TATase
-
Homo sapiens
tyrosine aminotransferase
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.25
-
2-oxoglutarate wild type enzyme Homo sapiens
0.3
-
2-oxoglutarate mutant enzyme I249A Homo sapiens
1
-
L-tyrosine wild type enzyme Homo sapiens
1.2
-
p-hydroxyphenylpyruvate wild type enzyme Homo sapiens
1.2
-
L-tyrosine mutant enzyme I249A Homo sapiens
3.8
-
L-glutamate wild type enzyme Homo sapiens
69
-
L-glutamate wild type enzyme Homo sapiens
83
-
L-tyrosine wild type enzyme Homo sapiens

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent Homo sapiens