Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.6.1.5 extracted from

  • Miller, J.E.; Litwack, G.
    Purification, properties, and identity of liver mitochondrial tyrosine aminotransferase (1971), J. Biol. Chem., 246, 3234-3240.
    View publication on PubMed
Search for more literature for 2.6.1.5

Crystallization (Commentary)

Crystallization (Comment) Organism
spontaneous crystallization when enzyme concentration is about 10 mg protein per ml Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
alpha-Methyl-L-aspartate with L-tyrosine and 2-oxoglutarate or oxaloacetate as substrates Rattus norvegicus
beta-Methyl-L-aspartate with L-tyrosine and 2-oxoglutarate or oxaloacetate as substrates Rattus norvegicus
D-Aspartate weak inhibition Rattus norvegicus
L-aspartate with L-tyrosine and 2-oxoglutarate or oxaloacetate as substrates Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 amino acid composition and gel filtration Rattus norvegicus
114000
-
 sedimentation analysis Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, using Sephadex chromatography and isoelectrofocusing Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.155
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(4-chlorophenyl)alanine + 2-oxoglutarate 17% of the activity with L-tyrosine Rattus norvegicus 3-(3-chlorophenyl)-2-oxopropanoate + L-glutamate
-
 ?
3,4-dihydroxyphenylalanine + 2-oxoglutarate 85% of the activity than with L-tyrosine Rattus norvegicus 3-(3,4-dihydroxyphenyl)-2-oxopropanoate + L-glutamate
-
 ?
3-iodotyrosine + 2-oxoglutarate 84% of the activity than with L-tyrosine Rattus norvegicus 3-(4-hydroxy-3-iodophenyl)-2-oxopropanoate + L-glutamate
-
 ?
3-methoxytyrosine + 2-oxoglutarate 22% of the activity with L-tyrosine Rattus norvegicus 3-(3-methoxy-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
 ?
4-hydroxyphenylpyruvate + L-aspartate 35% of the activity with L-glutamate Rattus norvegicus L-tyrosine + oxaloacetate
-
 r
L-asparagine + 2-oxoglutarate 15% of the activity with L-tyrosine Rattus norvegicus 2-oxosuccinamate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate higher activity than with L-tyrosine Rattus norvegicus oxaloacetate + L-glutamate
-
 ?
L-cysteine + 2-oxoglutarate higher activity than with L-tyrosine Rattus norvegicus 3-mercapto-2-oxopropanoate + L-glutamate
-
 ?
L-ethionine + 2-oxoglutarate 17% of the activity than with L-tyrosine Rattus norvegicus 4-ethylsulfanyl-2-oxobutanoate + L-glutamate
-
 ?
L-methionine + 2-oxoglutarate 20% of the activity than with L-tyrosine Rattus norvegicus 4-methylsulfanyl-2-oxobutanoate + L-glutamate
-
 ?
L-phenylalanine + 2-oxoglutarate higher rate than with L-tyrosine Rattus norvegicus phenylpyruvate + L-glutamate
-
 ?
L-tyrosine + 2-oxoglutarate
-
 Rattus norvegicus 4-hydroxyphenylpyruvate + L-glutamate
-
 r
L-tyrosine + oxaloacetate
-
 Rattus norvegicus 4-hydroxyphenylpyruvate + L-aspartate
-
 r
tryptophan + 2-oxoglutarate 62% of the activity than with L-tyrosine Rattus norvegicus 3-indole-2-oxopropanoate + L-glutamate
-
 ?

Synonyms

Synonyms Comment Organism
More the mitochondrial enzyme may be identical with EC 2.6.1.1 and with EC 2.6.1.57 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus