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Literature summary for 2.6.1.48 extracted from

  • Der Garabedian, P.A.
    Candida delta-aminovalerate: alpha-ketoglutarate aminotransferase: purification and enzymologic properties (1986), Biochemistry, 25, 5507-5512.
    View publication on PubMed
Search for more literature for 2.6.1.48

General Stability

General Stability Organism
2-oxoglutarate and pyridoxal 5'-phosphate protects against thermal denaturation Meyerozyma guilliermondii

Inhibitors

Inhibitors Comment Organism Structure
2,3-Diaminopropionate noncompetitive inhibition with respect to delta-aminovalerate, competitive inhibition to 2-oxoglutarate Meyerozyma guilliermondii
2,4-diaminobutyrate noncompetitive inhibition with respect to delta-aminovalerate, competitive inhibition to 2-oxoglutarate Meyerozyma guilliermondii
alanine noncompetitive inhibition with respect to delta-aminovalerate, competitive inhibition to 2-oxoglutarate Meyerozyma guilliermondii
beta-Alanine noncompetitive inhibition with respect to delta-aminovalerate, competitive inhibition to 2-oxoglutarate Meyerozyma guilliermondii
glycine noncompetitive inhibition with respect to delta-aminovalerate, competitive inhibition to 2-oxoglutarate Meyerozyma guilliermondii
L-lysine noncompetitive inhibition with respect to delta-aminovalerate, competitive inhibition to 2-oxoglutarate Meyerozyma guilliermondii
L-ornithine mixed inhibition with respect to delta-aminovalerate, competitive inhibition to 2-oxoglutarate Meyerozyma guilliermondii
additional information alpha-aminobutyrate, 4-aminobutyrate, alpha-aminovalerate and epsilon-aminocaproate does not cause any inhibition Meyerozyma guilliermondii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6
-
 2-oxoglutarate pH 7.8, 35°C Meyerozyma guilliermondii
4.9
-
 delta-aminovaleric acid pH 7.8, 35°C Meyerozyma guilliermondii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 2 * 60000, SDS-PAGE Meyerozyma guilliermondii
118000
-
 gel filtration Meyerozyma guilliermondii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-aminopentanoate + 2-oxoglutarate Meyerozyma guilliermondii enzyme is strongly induced in cells grown on delta-aminovalerate or on lysine as only nitrogen source 5-oxovalerate + L-glutamate
-
 r

Organism

Organism UniProt Comment Textmining
Meyerozyma guilliermondii
-
 var.membranaefaciens
-

Purification (Commentary)

Purification (Comment) Organism
-
 Meyerozyma guilliermondii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20.8
-
-
 Meyerozyma guilliermondii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-aminobutyrate + 2-oxoglutarate relative activity 40% Meyerozyma guilliermondii succinic semialdehyde + L-glutamate
-
 r
5-aminopentanoate + 2-oxoglutarate
-
 Meyerozyma guilliermondii 5-oxovalerate + L-glutamate
-
 r
5-aminopentanoate + 2-oxoglutarate enzyme is strongly induced in cells grown on delta-aminovalerate or on lysine as only nitrogen source Meyerozyma guilliermondii 5-oxovalerate + L-glutamate
-
 r
glycine + 2-oxoglutarate relative activity 1% Meyerozyma guilliermondii glyoxalate + L-glutamate
-
 r
L-leucine + 2-oxoglutarate relative activity 10% Meyerozyma guilliermondii 4-methyl-2-oxopentanoate + L-glutamate
-
 r
L-norleucine + 2-oxoglutarate relative activity 4.8% Meyerozyma guilliermondii 2-oxohexanoate + L-glutamate
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 60000, SDS-PAGE Meyerozyma guilliermondii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Meyerozyma guilliermondii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 preserves 50% of its activity after being heated for 7 min, in presence of 2-oxoglutarate Meyerozyma guilliermondii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8.5
-
 Meyerozyma guilliermondii

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate Km 0.0227 mM Meyerozyma guilliermondii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5
-
 beta-Alanine pH 7.8, 35°C, competitive inhibition with respect to 2-oxoglutarate Meyerozyma guilliermondii
5.6
-
 L-ornithine pH 7.8, 35°C, competitive inhibition with respect to 2-oxoglutarate Meyerozyma guilliermondii
7
-
 2,3-Diaminopropionate pH 7.8, 35°C, competitive inhibition with respect to 2-oxoglutarate Meyerozyma guilliermondii
9.5
-
 ornithine pH 7.8, 35°C, mixed inhibition Meyerozyma guilliermondii
10
-
 glycine pH 7.8, 35°C, competitive inhibition with respect to 2-oxoglutarate Meyerozyma guilliermondii
11.5
-
 2,4-diaminobutyrate pH 7.8, 35°C, competitive inhibition with respect to 2-oxoglutarate Meyerozyma guilliermondii
12.5
-
 L-lysine pH 7.8, 35°C, competitive inhibition with respect to 2-oxoglutarate Meyerozyma guilliermondii
15
-
 alanine pH 7.8, 35°C, competitive inhibition with respect to 2-oxoglutarate Meyerozyma guilliermondii
16
-
 beta-Alanine pH 7.8, 35°C, noncompetitive inhibition with respect to delta-aminovalerate Meyerozyma guilliermondii
50
-
 2,3-Diaminopropionate pH 7.8, 35°C, noncompetitive inhibition with respect to delta-aminovalerate Meyerozyma guilliermondii
90
-
 glycine pH 7.8, 35°C, noncompetitive inhibition with respect to delta-aminovalerate Meyerozyma guilliermondii
120
-
 alanine pH 7.8, 35°C, noncompetitive inhibition with respect to delta-aminovalerate Meyerozyma guilliermondii
120
-
 lysine pH 7.8, 35°C, noncompetitive inhibition with respect to delta-aminovalerate Meyerozyma guilliermondii
190
-
 2,4-diaminobutyrate pH 7.8, 35°C, noncompetitive inhibition with respect to delta-aminovalerate Meyerozyma guilliermondii