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Literature summary for 2.6.1.46 extracted from

  • Peters, P.; Galinski, E.A.; Trüper, H.G.
    The biosynthesis of ectoine (1990), FEMS Microbiol. Lett., 71, 157-162.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2
-
L-glutamate pH 9.1, 37ºC Halorhodospira halochloris
1.85
-
L-glutamate
-
Halomonas elongata
1.85
-
L-glutamate pH 9.1, 37ºC Halorhodospira halochloris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-2-amino-4-oxobutanoate + L-glutamate Halorhodospira halochloris involved in the biosynthesis of the osmolyte ectoine in halophilic eubacteria L-2,4-diaminobutanoate + 2-oxoglutarate
-
?
L-2-amino-4-oxobutanoate + L-glutamate Halomonas elongata involved in the biosynthesis of the osmolyte ectoine in halophilic eubacteria L-2,4-diaminobutanoate + 2-oxoglutarate
-
?

Organism

Organism UniProt Comment Textmining
Halomonas elongata
-
strain DSM 2581
-
Halorhodospira halochloris
-
strain DSM 1059
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.054
-
-
Halorhodospira halochloris
0.171
-
-
Halomonas elongata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-2-amino-4-oxobutanoate + L-glutamate
-
Halorhodospira halochloris L-2,4-diaminobutanoate + 2-oxoglutarate
-
?
L-2-amino-4-oxobutanoate + L-glutamate
-
Halomonas elongata L-2,4-diaminobutanoate + 2-oxoglutarate
-
?
L-2-amino-4-oxobutanoate + L-glutamate involved in the biosynthesis of the osmolyte ectoine in halophilic eubacteria Halorhodospira halochloris L-2,4-diaminobutanoate + 2-oxoglutarate
-
?
L-2-amino-4-oxobutanoate + L-glutamate involved in the biosynthesis of the osmolyte ectoine in halophilic eubacteria Halomonas elongata L-2,4-diaminobutanoate + 2-oxoglutarate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Halorhodospira halochloris
37
-
assay at Halomonas elongata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
-
Halomonas elongata
8.2
-
-
Halorhodospira halochloris