Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta (DE3) | Methylosinus trichosporium |
DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta (DE3) | Methylotuvimicrobium alcaliphilum |
DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta (DE3) | Methylococcus capsulatus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the sga20Z gene is knocked out by the insertion of the kanamycin cassette using double homologous recombination. The growth rate of the DELTAsga strain on methane is significantly lower as compared to the wild-type strain. During growth on methanol, this effect is even more pronounced | Methylotuvimicrobium alcaliphilum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | 2-oxoglutarate at a concentration above 12.5 mM inhibits enzyme SgaBath in the serine-2-oxoglutarate aminotransferase (SKAT) reaction, but has no effect on the serine-glyoxylate aminotranferase (SGAT) reaction, substrate inhibition | Methylococcus capsulatus | |
2-oxoglutarate | 2-oxoglutarate at a concentration above 12.5 mM inhibits enzyme Sga20Z in the serine-2-oxoglutarate aminotransferase (SKAT) reaction, but has no effect on the serine-glyoxylate aminotranferase (SGAT) reaction, substrate inhibition | Methylotuvimicrobium alcaliphilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.089 | - |
L-serine | pH 7.5, temperature not specified in the publication, recombinant enzyme, with pyruvate | Methylotuvimicrobium alcaliphilum | |
0.089 | - |
L-serine | pH 7.5, temperature not specified in the publication, recombinant enzyme, with pyruvate | Methylococcus capsulatus | |
0.17 | - |
glyoxylate | pH 7.0, temperature not specified in the publication, recombinant enzyme, with L-serine | Methylosinus trichosporium | |
0.18 | - |
L-serine | pH 7.0, temperature not specified in the publication, recombinant enzyme, with pyruvate | Methylosinus trichosporium | |
0.24 | - |
glyoxylate | pH 7.5, temperature not specified in the publication, recombinant enzyme, with L-serine | Methylotuvimicrobium alcaliphilum | |
0.59 | - |
glyoxylate | pH 7.5, temperature not specified in the publication, recombinant enzyme, with L-alanine | Methylococcus capsulatus | |
0.7 | - |
glyoxylate | pH 7.5, temperature not specified in the publication, recombinant enzyme, with L-serine | Methylococcus capsulatus | |
1 | - |
glyoxylate | pH 7.5, temperature not specified in the publication, recombinant enzyme, with L-alanine | Methylotuvimicrobium alcaliphilum | |
1.06 | - |
L-serine | pH 7.5, temperature not specified in the publication, recombinant enzyme, with 2-oxoglutarate | Methylococcus capsulatus | |
1.17 | - |
L-serine | pH 7.5, temperature not specified in the publication, recombinant enzyme, with 2-oxoglutarate | Methylotuvimicrobium alcaliphilum | |
1.62 | - |
L-serine | pH 7.5, temperature not specified in the publication, recombinant enzyme, with glyoxylate | Methylococcus capsulatus | |
2 | 3 | L-alanine | pH 7.5, temperature not specified in the publication, recombinant enzyme, with glyoxylate | Methylococcus capsulatus | |
2.1 | - |
L-serine | pH 7.0, temperature not specified in the publication, recombinant enzyme, with glyoxylate | Methylosinus trichosporium | |
2.6 | - |
L-serine | pH 7.5, temperature not specified in the publication, recombinant enzyme, with glyoxylate | Methylotuvimicrobium alcaliphilum | |
4.3 | - |
2-oxoglutarate | pH 7.5, temperature not specified in the publication, recombinant enzyme, with L-serine | Methylococcus capsulatus | |
4.5 | - |
pyruvate | pH 7.5, temperature not specified in the publication, recombinant enzyme, with L-serine | Methylococcus capsulatus | |
5 | - |
2-oxoglutarate | pH 7.5, temperature not specified in the publication, recombinant enzyme, with L-serine | Methylotuvimicrobium alcaliphilum | |
5.8 | - |
pyruvate | pH 7.0, temperature not specified in the publication, recombinant enzyme, with L-serine | Methylosinus trichosporium | |
6 | - |
pyruvate | pH 7.5, temperature not specified in the publication, recombinant enzyme, with L-serine | Methylotuvimicrobium alcaliphilum | |
14 | - |
L-alanine | pH 7.5, temperature not specified in the publication, recombinant enzyme, with glyoxylate | Methylotuvimicrobium alcaliphilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
gel filtration | Methylotuvimicrobium alcaliphilum |
120000 | - |
gel filtration | Methylococcus capsulatus |
170000 | - |
gel filtration | Methylosinus trichosporium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + glyoxylate | Methylotuvimicrobium alcaliphilum | reaction of alanine-glyoxylate aminotransferase (AGAT), EC 2.6.1.44 | pyruvate + glycine | - |
? | |
L-alanine + glyoxylate | Methylococcus capsulatus | reaction of alanine-glyoxylate aminotransferase (AGAT), EC 2.6.1.44 | pyruvate + glycine | - |
? | |
L-alanine + glyoxylate | Methylococcus capsulatus Bath | reaction of alanine-glyoxylate aminotransferase (AGAT), EC 2.6.1.44 | pyruvate + glycine | - |
? | |
L-alanine + glyoxylate | Methylotuvimicrobium alcaliphilum VKM B-2133 | reaction of alanine-glyoxylate aminotransferase (AGAT), EC 2.6.1.44 | pyruvate + glycine | - |
? | |
L-alanine + glyoxylate | Methylococcus capsulatus ATCC 33009 | reaction of alanine-glyoxylate aminotransferase (AGAT), EC 2.6.1.44 | pyruvate + glycine | - |
? | |
L-alanine + glyoxylate | Methylotuvimicrobium alcaliphilum 20Z | reaction of alanine-glyoxylate aminotransferase (AGAT), EC 2.6.1.44 | pyruvate + glycine | - |
? | |
L-alanine + glyoxylate | Methylococcus capsulatus NCIMB 11132 | reaction of alanine-glyoxylate aminotransferase (AGAT), EC 2.6.1.44 | pyruvate + glycine | - |
? | |
L-alanine + glyoxylate | Methylotuvimicrobium alcaliphilum DSM 19304 | reaction of alanine-glyoxylate aminotransferase (AGAT), EC 2.6.1.44 | pyruvate + glycine | - |
? | |
L-alanine + glyoxylate | Methylotuvimicrobium alcaliphilum NCIMB 14124 | reaction of alanine-glyoxylate aminotransferase (AGAT), EC 2.6.1.44 | pyruvate + glycine | - |
? | |
L-serine + 2-oxoglutarate | Methylotuvimicrobium alcaliphilum | - |
3-hydroxypyruvate + L-glutamate | - |
? | |
L-serine + 2-oxoglutarate | Methylococcus capsulatus | - |
3-hydroxypyruvate + L-glutamate | - |
? | |
L-serine + 2-oxoglutarate | Methylococcus capsulatus Bath | - |
3-hydroxypyruvate + L-glutamate | - |
? | |
L-serine + 2-oxoglutarate | Methylotuvimicrobium alcaliphilum VKM B-2133 | - |
3-hydroxypyruvate + L-glutamate | - |
? | |
L-serine + 2-oxoglutarate | Methylococcus capsulatus ATCC 33009 | - |
3-hydroxypyruvate + L-glutamate | - |
? | |
L-serine + 2-oxoglutarate | Methylotuvimicrobium alcaliphilum 20Z | - |
3-hydroxypyruvate + L-glutamate | - |
? | |
L-serine + 2-oxoglutarate | Methylococcus capsulatus NCIMB 11132 | - |
3-hydroxypyruvate + L-glutamate | - |
? | |
L-serine + 2-oxoglutarate | Methylotuvimicrobium alcaliphilum DSM 19304 | - |
3-hydroxypyruvate + L-glutamate | - |
? | |
L-serine + 2-oxoglutarate | Methylotuvimicrobium alcaliphilum NCIMB 14124 | - |
3-hydroxypyruvate + L-glutamate | - |
? | |
L-serine + glyoxylate | Methylosinus trichosporium | preferred substrates | 3-hydroxypyruvate + glycine | - |
? | |
L-serine + glyoxylate | Methylotuvimicrobium alcaliphilum | preferred substrates | 3-hydroxypyruvate + glycine | - |
? | |
L-serine + glyoxylate | Methylococcus capsulatus | preferred substrates | 3-hydroxypyruvate + glycine | - |
? | |
L-serine + glyoxylate | Methylococcus capsulatus Bath | preferred substrates | 3-hydroxypyruvate + glycine | - |
? | |
L-serine + glyoxylate | Methylotuvimicrobium alcaliphilum VKM B-2133 | preferred substrates | 3-hydroxypyruvate + glycine | - |
? | |
L-serine + glyoxylate | Methylococcus capsulatus ATCC 33009 | preferred substrates | 3-hydroxypyruvate + glycine | - |
? | |
L-serine + glyoxylate | Methylotuvimicrobium alcaliphilum 20Z | preferred substrates | 3-hydroxypyruvate + glycine | - |
? | |
L-serine + glyoxylate | Methylococcus capsulatus NCIMB 11132 | preferred substrates | 3-hydroxypyruvate + glycine | - |
? | |
L-serine + glyoxylate | Methylotuvimicrobium alcaliphilum DSM 19304 | preferred substrates | 3-hydroxypyruvate + glycine | - |
? | |
L-serine + glyoxylate | Methylotuvimicrobium alcaliphilum NCIMB 14124 | preferred substrates | 3-hydroxypyruvate + glycine | - |
? | |
L-serine + pyruvate | Methylosinus trichosporium | reaction of serine-pyruvate aminotransferase (SPAT), EC 2.6.1.51 | 3-hydroxypyruvate + L-alanine | - |
? | |
L-serine + pyruvate | Methylotuvimicrobium alcaliphilum | reaction of serine-pyruvate aminotransferase (SPAT), EC 2.6.1.51 | 3-hydroxypyruvate + L-alanine | - |
? | |
L-serine + pyruvate | Methylococcus capsulatus | reaction of serine-pyruvate aminotransferase (SPAT), EC 2.6.1.51 | 3-hydroxypyruvate + L-alanine | - |
? | |
L-serine + pyruvate | Methylococcus capsulatus Bath | reaction of serine-pyruvate aminotransferase (SPAT), EC 2.6.1.51 | 3-hydroxypyruvate + L-alanine | - |
? | |
L-serine + pyruvate | Methylotuvimicrobium alcaliphilum VKM B-2133 | reaction of serine-pyruvate aminotransferase (SPAT), EC 2.6.1.51 | 3-hydroxypyruvate + L-alanine | - |
? | |
L-serine + pyruvate | Methylococcus capsulatus ATCC 33009 | reaction of serine-pyruvate aminotransferase (SPAT), EC 2.6.1.51 | 3-hydroxypyruvate + L-alanine | - |
? | |
L-serine + pyruvate | Methylotuvimicrobium alcaliphilum 20Z | reaction of serine-pyruvate aminotransferase (SPAT), EC 2.6.1.51 | 3-hydroxypyruvate + L-alanine | - |
? | |
L-serine + pyruvate | Methylococcus capsulatus NCIMB 11132 | reaction of serine-pyruvate aminotransferase (SPAT), EC 2.6.1.51 | 3-hydroxypyruvate + L-alanine | - |
? | |
L-serine + pyruvate | Methylotuvimicrobium alcaliphilum DSM 19304 | reaction of serine-pyruvate aminotransferase (SPAT), EC 2.6.1.51 | 3-hydroxypyruvate + L-alanine | - |
? | |
L-serine + pyruvate | Methylotuvimicrobium alcaliphilum NCIMB 14124 | reaction of serine-pyruvate aminotransferase (SPAT), EC 2.6.1.51 | 3-hydroxypyruvate + L-alanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylococcus capsulatus | Q608T3 | - |
- |
Methylococcus capsulatus ATCC 33009 | Q608T3 | - |
- |
Methylococcus capsulatus Bath | Q608T3 | - |
- |
Methylococcus capsulatus NCIMB 11132 | Q608T3 | - |
- |
Methylosinus trichosporium | - |
- |
- |
Methylotuvimicrobium alcaliphilum | G4T3S7 | - |
- |
Methylotuvimicrobium alcaliphilum 20Z | G4T3S7 | - |
- |
Methylotuvimicrobium alcaliphilum DSM 19304 | G4T3S7 | - |
- |
Methylotuvimicrobium alcaliphilum NCIMB 14124 | G4T3S7 | - |
- |
Methylotuvimicrobium alcaliphilum VKM B-2133 | G4T3S7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography | Methylosinus trichosporium |
recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography | Methylotuvimicrobium alcaliphilum |
recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography | Methylococcus capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + glyoxylate | reaction of alanine-glyoxylate aminotransferase (AGAT), EC 2.6.1.44 | Methylotuvimicrobium alcaliphilum | pyruvate + glycine | - |
? | |
L-alanine + glyoxylate | reaction of alanine-glyoxylate aminotransferase (AGAT), EC 2.6.1.44 | Methylococcus capsulatus | pyruvate + glycine | - |
? | |
L-alanine + glyoxylate | reaction of alanine-glyoxylate aminotransferase (AGAT), EC 2.6.1.44 | Methylococcus capsulatus Bath | pyruvate + glycine | - |
? | |
L-alanine + glyoxylate | reaction of alanine-glyoxylate aminotransferase (AGAT), EC 2.6.1.44 | Methylotuvimicrobium alcaliphilum VKM B-2133 | pyruvate + glycine | - |
? | |
L-alanine + glyoxylate | reaction of alanine-glyoxylate aminotransferase (AGAT), EC 2.6.1.44 | Methylococcus capsulatus ATCC 33009 | pyruvate + glycine | - |
? | |
L-alanine + glyoxylate | reaction of alanine-glyoxylate aminotransferase (AGAT), EC 2.6.1.44 | Methylotuvimicrobium alcaliphilum 20Z | pyruvate + glycine | - |
? | |
L-alanine + glyoxylate | reaction of alanine-glyoxylate aminotransferase (AGAT), EC 2.6.1.44 | Methylococcus capsulatus NCIMB 11132 | pyruvate + glycine | - |
? | |
L-alanine + glyoxylate | reaction of alanine-glyoxylate aminotransferase (AGAT), EC 2.6.1.44 | Methylotuvimicrobium alcaliphilum DSM 19304 | pyruvate + glycine | - |
? | |
L-alanine + glyoxylate | reaction of alanine-glyoxylate aminotransferase (AGAT), EC 2.6.1.44 | Methylotuvimicrobium alcaliphilum NCIMB 14124 | pyruvate + glycine | - |
? | |
L-serine + 2-oxoglutarate | - |
Methylotuvimicrobium alcaliphilum | 3-hydroxypyruvate + L-glutamate | - |
? | |
L-serine + 2-oxoglutarate | - |
Methylococcus capsulatus | 3-hydroxypyruvate + L-glutamate | - |
? | |
L-serine + 2-oxoglutarate | - |
Methylococcus capsulatus Bath | 3-hydroxypyruvate + L-glutamate | - |
? | |
L-serine + 2-oxoglutarate | - |
Methylotuvimicrobium alcaliphilum VKM B-2133 | 3-hydroxypyruvate + L-glutamate | - |
? | |
L-serine + 2-oxoglutarate | - |
Methylococcus capsulatus ATCC 33009 | 3-hydroxypyruvate + L-glutamate | - |
? | |
L-serine + 2-oxoglutarate | - |
Methylotuvimicrobium alcaliphilum 20Z | 3-hydroxypyruvate + L-glutamate | - |
? | |
L-serine + 2-oxoglutarate | - |
Methylococcus capsulatus NCIMB 11132 | 3-hydroxypyruvate + L-glutamate | - |
? | |
L-serine + 2-oxoglutarate | - |
Methylotuvimicrobium alcaliphilum DSM 19304 | 3-hydroxypyruvate + L-glutamate | - |
? | |
L-serine + 2-oxoglutarate | - |
Methylotuvimicrobium alcaliphilum NCIMB 14124 | 3-hydroxypyruvate + L-glutamate | - |
? | |
L-serine + glyoxylate | preferred substrates | Methylosinus trichosporium | 3-hydroxypyruvate + glycine | - |
? | |
L-serine + glyoxylate | preferred substrates | Methylotuvimicrobium alcaliphilum | 3-hydroxypyruvate + glycine | - |
? | |
L-serine + glyoxylate | preferred substrates | Methylococcus capsulatus | 3-hydroxypyruvate + glycine | - |
? | |
L-serine + glyoxylate | preferred substrates | Methylococcus capsulatus Bath | 3-hydroxypyruvate + glycine | - |
? | |
L-serine + glyoxylate | preferred substrates | Methylotuvimicrobium alcaliphilum VKM B-2133 | 3-hydroxypyruvate + glycine | - |
? | |
L-serine + glyoxylate | preferred substrates | Methylococcus capsulatus ATCC 33009 | 3-hydroxypyruvate + glycine | - |
? | |
L-serine + glyoxylate | preferred substrates | Methylotuvimicrobium alcaliphilum 20Z | 3-hydroxypyruvate + glycine | - |
? | |
L-serine + glyoxylate | preferred substrates | Methylococcus capsulatus NCIMB 11132 | 3-hydroxypyruvate + glycine | - |
? | |
L-serine + glyoxylate | preferred substrates | Methylotuvimicrobium alcaliphilum DSM 19304 | 3-hydroxypyruvate + glycine | - |
? | |
L-serine + glyoxylate | preferred substrates | Methylotuvimicrobium alcaliphilum NCIMB 14124 | 3-hydroxypyruvate + glycine | - |
? | |
L-serine + pyruvate | reaction of serine-pyruvate aminotransferase (SPAT), EC 2.6.1.51 | Methylosinus trichosporium | 3-hydroxypyruvate + L-alanine | - |
? | |
L-serine + pyruvate | reaction of serine-pyruvate aminotransferase (SPAT), EC 2.6.1.51 | Methylotuvimicrobium alcaliphilum | 3-hydroxypyruvate + L-alanine | - |
? | |
L-serine + pyruvate | reaction of serine-pyruvate aminotransferase (SPAT), EC 2.6.1.51 | Methylococcus capsulatus | 3-hydroxypyruvate + L-alanine | - |
? | |
L-serine + pyruvate | reaction of serine-pyruvate aminotransferase (SPAT), EC 2.6.1.51 | Methylococcus capsulatus Bath | 3-hydroxypyruvate + L-alanine | - |
? | |
L-serine + pyruvate | reaction of serine-pyruvate aminotransferase (SPAT), EC 2.6.1.51 | Methylotuvimicrobium alcaliphilum VKM B-2133 | 3-hydroxypyruvate + L-alanine | - |
? | |
L-serine + pyruvate | reaction of serine-pyruvate aminotransferase (SPAT), EC 2.6.1.51 | Methylococcus capsulatus ATCC 33009 | 3-hydroxypyruvate + L-alanine | - |
? | |
L-serine + pyruvate | reaction of serine-pyruvate aminotransferase (SPAT), EC 2.6.1.51 | Methylotuvimicrobium alcaliphilum 20Z | 3-hydroxypyruvate + L-alanine | - |
? | |
L-serine + pyruvate | reaction of serine-pyruvate aminotransferase (SPAT), EC 2.6.1.51 | Methylococcus capsulatus NCIMB 11132 | 3-hydroxypyruvate + L-alanine | - |
? | |
L-serine + pyruvate | reaction of serine-pyruvate aminotransferase (SPAT), EC 2.6.1.51 | Methylotuvimicrobium alcaliphilum DSM 19304 | 3-hydroxypyruvate + L-alanine | - |
? | |
L-serine + pyruvate | reaction of serine-pyruvate aminotransferase (SPAT), EC 2.6.1.51 | Methylotuvimicrobium alcaliphilum NCIMB 14124 | 3-hydroxypyruvate + L-alanine | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 4 * 45000, SDS-PAGE | Methylosinus trichosporium |
homodimer | 2 * 40000, SDS-PAGE | Methylotuvimicrobium alcaliphilum |
homotrimer | 3 * 40000, SDS-PAGE | Methylococcus capsulatus |
Synonyms | Comment | Organism |
---|---|---|
More | see also EC 2.6.1.44 and EC 2.6.1.51 | Methylotuvimicrobium alcaliphilum |
More | see also EC 2.6.1.51 | Methylosinus trichosporium |
More | see also EC 2.6.1.51 and 2.6.1.44 | Methylococcus capsulatus |
serine-alpha-ketoglutarate aminotransferase | - |
Methylotuvimicrobium alcaliphilum |
serine-alpha-ketoglutarate aminotransferase | - |
Methylococcus capsulatus |
serine-glyoxylate aminotranferase | - |
Methylosinus trichosporium |
serine-glyoxylate aminotranferase | - |
Methylococcus capsulatus |
serine-glyoxylate aminotransferase | - |
Methylotuvimicrobium alcaliphilum |
Sga | - |
Methylosinus trichosporium |
Sga | - |
Methylotuvimicrobium alcaliphilum |
Sga | - |
Methylococcus capsulatus |
Sga20Z | - |
Methylotuvimicrobium alcaliphilum |
SgaBath | - |
Methylococcus capsulatus |
SGAT | - |
Methylotuvimicrobium alcaliphilum |
SGAT | - |
Methylococcus capsulatus |
SKAT | - |
Methylotuvimicrobium alcaliphilum |
SKAT | - |
Methylococcus capsulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Methylosinus trichosporium |
7 | - |
- |
Methylococcus capsulatus |
7.5 | - |
- |
Methylotuvimicrobium alcaliphilum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | PLP | Methylosinus trichosporium | |
pyridoxal 5'-phosphate | PLP | Methylotuvimicrobium alcaliphilum | |
pyridoxal 5'-phosphate | PLP | Methylococcus capsulatus |
General Information | Comment | Organism |
---|---|---|
evolution | phylogenetic analysis and tree | Methylosinus trichosporium |
evolution | phylogenetic analysis and tree | Methylotuvimicrobium alcaliphilum |
evolution | phylogenetic analysis and tree | Methylococcus capsulatus |
malfunction | the disruption of the sga gene in Methylomicrobium alcaliphilum results in retardation of growth rate of the mutant cells and in a prolonged lag-phase after passaging from methane to methanol. In addition, the growth of the mutant strain is accompanied by formaldehyde accumulation in the culture liquid | Methylotuvimicrobium alcaliphilum |
metabolism | serine-glyoxylate aminotransferase (Sga) is the indicator enzyme of the serine pathway of assimilation of reduced C1 compounds | Methylosinus trichosporium |
metabolism | serine-glyoxylate aminotransferase (Sga) is the indicator enzyme of the serine pathway of assimilation of reduced C1 compounds | Methylotuvimicrobium alcaliphilum |
metabolism | serine-glyoxylate aminotransferase (Sga) is the indicator enzyme of the serine pathway of assimilation of reduced C1 compounds | Methylococcus capsulatus |
additional information | influence of metabolites on the activity of recombinant serineglyoxylate aminotransferase, overview | Methylosinus trichosporium |
additional information | influence of metabolites on the activity of recombinant serineglyoxylate aminotransferase, overview | Methylotuvimicrobium alcaliphilum |
additional information | influence of metabolites on the activity of recombinant serineglyoxylate aminotransferase, overview | Methylococcus capsulatus |
physiological function | serine-glyoxylate aminotransferase (Sga) is the indicator enzyme of the serine pathway of assimilation of reduced C1 compounds. The Sga enzyme from the methanotroph catalyses the pyridoxal phosphate-dependent transfer of an amino group from serine to glyoxylate and pyruvate. Enzyme Sga is important in the serine cycle of type I methanotrophs and this pathway might be related to the removal of excess formaldehyde and/or energy regulation | Methylosinus trichosporium |
physiological function | serine-glyoxylate aminotransferase (Sga) is the indicator enzyme of the serine pathway of assimilation of reduced C1 compounds. The Sga enzyme from the methanotroph catalyses the pyridoxal phosphate-dependent transfer of an amino group from serine to glyoxylate and pyruvate. The enzyme also transfers an amino group from serine to 2-oxoglutarate and from alanine to glyoxylate. Enzyme Sga is important in the serine cycle of type I methanotrophs and this pathway might be related to the removal of excess formaldehyde and/or energy regulation | Methylotuvimicrobium alcaliphilum |
physiological function | serine-glyoxylate aminotransferase (Sga) is the indicator enzyme of the serine pathway of assimilation of reduced C1 compounds. The Sga enzyme from the methanotroph catalyses the pyridoxal phosphate-dependent transfer of an amino group from serine to glyoxylate and pyruvate. The enzyme also transfers an amino group from serine to 2-oxoglutarate and from alanine to glyoxylate. Enzyme Sga is important in the serine cycle of type I methanotrophs and this pathway might be related to the removal of excess formaldehyde and/or energy regulation | Methylococcus capsulatus |