Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.6.1.45 extracted from

  • Hagishita, T.; Yoshida, T.; Izumi, Y.; Mitsunaga, T.
    Cloning and expression of the gene for serine-glyoxylate aminotransferase from an obligate methylotroph Hyphomicrobium methylovorum GM2 (1996), Eur. J. Biochem., 241, 1-5.
    View publication on PubMed
Search for more literature for 2.6.1.45

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Hyphomicrobium methylovorum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.68
-
 glyoxylate recombinant enzyme Hyphomicrobium methylovorum
3.86
-
 L-serine recombinant enzyme Hyphomicrobium methylovorum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 4 * 40000, recombinant enzyme, SDS-PAGE Hyphomicrobium methylovorum

Organism

Organism UniProt Comment Textmining
Hyphomicrobium methylovorum
-
 GM2, glycine-resistant mutant derived from parent strain KM146
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Hyphomicrobium methylovorum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
72
-
 recombinant enzyme Hyphomicrobium methylovorum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine + glyoxylate
-
 Hyphomicrobium methylovorum 3-hydroxypyruvate + glycine
-
 ir

Subunits

Subunits Comment Organism
tetramer 4 * 40000, recombinant enzyme, SDS-PAGE Hyphomicrobium methylovorum