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Literature summary for 2.6.1.45 extracted from

  • Izumi, Y.; Yoshida, T.; Yamada, H.
    Purification and characterization of serine-glyoxylate aminotransferase from a serine-producing methylotroph, Hyphomicrobium methylovorum GM2 (1990), Eur. J. Biochem., 190, 285-290.
    View publication on PubMed
Search for more literature for 2.6.1.45

Inhibitors

Inhibitors Comment Organism Structure
3-chloro-L-alanine
-
 Hyphomicrobium methylovorum
AgNO3 strong inhibition Hyphomicrobium methylovorum
cycloserine weak inhibition Hyphomicrobium methylovorum
HgCl2 strong inhibition Hyphomicrobium methylovorum
hydroxylamine
-
 Hyphomicrobium methylovorum
iodoacetamide
-
 Hyphomicrobium methylovorum
KCN weak inhibition Hyphomicrobium methylovorum
MgCl2 weak inhibition Hyphomicrobium methylovorum
additional information not inhibited by NaBH4, 3-chloro-D-alanine, 1,10-phenanthroline, 2,2'-dipyridyl, 8-hydroxyquinoline, EDTA, iodoacetate, FeCl3, AlCl3, CdCl2, CoCl2, NiCl2, CaCl2, NaN3, ZnCl2, PbCl2, LiCl, CuCl2, BaCl2, ascorbate, cysteamine, N-ethylmaleimide Hyphomicrobium methylovorum
NaF weak inhibition Hyphomicrobium methylovorum
p-chloromercuribenzoate
-
 Hyphomicrobium methylovorum
Penicillamine weak inhibition Hyphomicrobium methylovorum
phenylhydrazine weak inhibition Hyphomicrobium methylovorum
Semicarbazide
-
 Hyphomicrobium methylovorum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.23
-
 glyoxylate pH 7.0, 30°C Hyphomicrobium methylovorum
4.98
-
 L-serine
-
 Hyphomicrobium methylovorum

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Hyphomicrobium methylovorum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 4 * 40000, SDS-PAGE Hyphomicrobium methylovorum
140000
-
 HPLC, gel filtration Hyphomicrobium methylovorum
150000
-
 Sephacryl S-200, gel filtration Hyphomicrobium methylovorum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-serine + glyoxylate Hyphomicrobium methylovorum plays essential role in methanol assimilation through serine pathway 3-hydroxypyruvate + glycine
-
 ?

Organism

Organism UniProt Comment Textmining
Hyphomicrobium methylovorum
-
 GM2, glycine-resistant mutant derived from parent strain KM146
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-Sephacel, phenyl-Sepharose, CM-toyopearl, Cellulofine Hyphomicrobium methylovorum

Reaction

Reaction Comment Organism Reaction ID
L-serine + glyoxylate = 3-hydroxypyruvate + glycine ping-pong reaction mechanism Hyphomicrobium methylovorum
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
69.5
-
-
 Hyphomicrobium methylovorum

Storage Stability

Storage Stability Organism
-20°C, 10 mM phosphate buffer pH 7, 0.1 mM pyridoxal phosphate, 0.1 mM dithiothreitol, 45% v/v glycerol, at least 5 months Hyphomicrobium methylovorum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine + 2-oxomalonate
-
 Hyphomicrobium methylovorum 3-hydroxypyruvate + 2-aminomalonate
-
 ?
L-serine + glyoxylate highly specific Hyphomicrobium methylovorum 3-hydroxypyruvate + glycine
-
 ir
L-serine + glyoxylate no activity with Phe, Arg, Val, Trp, Thr, Met Hyphomicrobium methylovorum 3-hydroxypyruvate + glycine
-
 ir
L-serine + glyoxylate nearly irreversible, trace amounts of L-serine Hyphomicrobium methylovorum 3-hydroxypyruvate + glycine
-
 ir
L-serine + glyoxylate no activity with D-serine Hyphomicrobium methylovorum 3-hydroxypyruvate + glycine
-
 ir
L-serine + glyoxylate no or trace activity with oxaloacetate, 2-oxoglutarate Hyphomicrobium methylovorum 3-hydroxypyruvate + glycine
-
 ir
L-serine + glyoxylate no activity with Ala, Glu, Gln, His, oxamate, 2-oxo-n-butanoate, 3-methyl-2-oxo-butanoate, 2-methyl-DL-serine, L-serine-O-sulfate, DL-serine hydroxamate, O-phospho-L-serine Hyphomicrobium methylovorum 3-hydroxypyruvate + glycine
-
 ir
L-serine + glyoxylate plays essential role in methanol assimilation through serine pathway Hyphomicrobium methylovorum 3-hydroxypyruvate + glycine
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 40000, SDS-PAGE Hyphomicrobium methylovorum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Hyphomicrobium methylovorum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 and below, 30 min stable Hyphomicrobium methylovorum
40
-
 30 min, 16% loss of activity Hyphomicrobium methylovorum
45
-
 52% loss of activity after 30 min Hyphomicrobium methylovorum
50
-
 30 min, 93% loss of activity Hyphomicrobium methylovorum
55
-
 30 min, inactivation Hyphomicrobium methylovorum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pI: 6.9 Hyphomicrobium methylovorum
8
-
-
 Hyphomicrobium methylovorum

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 11 stable for 30 min at 30°C Hyphomicrobium methylovorum

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate required for activity Hyphomicrobium methylovorum
pyridoxal 5'-phosphate a pyridoxal phosphate protein, 4 mol per mol enzyme Hyphomicrobium methylovorum