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Literature summary for 2.6.1.44 extracted from

  • Cellini, B.; Montioli, R.; Paiardini, A.; Lorenzetto, A.; Voltattorni, C.B.
    Molecular insight into the synergism between the minor allele of human liver peroxisomal alanine:glyoxylate aminotransferase and the F152I mutation (2009), J. Biol. Chem., 284, 8349-8358.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Homo sapiens

Protein Variants

Protein Variants Comment Organism
F152A the mutant shows decreased activity compared to the wild type enzyme Homo sapiens
F152I the mutation is associated with primary hyperoxaluria type 1 in combination with the minor AGT allele and shows decreased activity compared to the wild type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
glyoxylate minor allele wild type enzyme Homo sapiens
0.23
-
glyoxylate major allele wild type enzyme Homo sapiens
0.25
-
glyoxylate minor allele mutant enzyme F152I Homo sapiens
0.28
-
glyoxylate major allele wild mutant enzyme F152I Homo sapiens
0.34
-
glyoxylate major allele wild mutant enzyme F152A Homo sapiens
28
-
L-alanine minor allele wild type enzyme Homo sapiens
31
-
L-alanine major allele wild type enzyme Homo sapiens
37
-
L-alanine major allele mutant enzyme F152I Homo sapiens
41
-
L-alanine minor allele mutant enzyme F152I Homo sapiens
46
-
L-alanine major allele mutant enzyme F152A Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
Homo sapiens 5777
-

Organism

Organism UniProt Comment Textmining
Homo sapiens P21549
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine + glyoxylate
-
Homo sapiens pyruvate + glycine
-
?

Synonyms

Synonyms Comment Organism
AGT
-
Homo sapiens
AGT-Ma major allele of alanine:glyoxylate aminotransferase Homo sapiens
AGT-Mi minor allele of alanine:glyoxylate aminotransferase Homo sapiens
alanine:glyoxylate aminotransferase
-
Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70.1
-
the melting temperature of the minor allele holoenzyme is at 70.1°C Homo sapiens
76.3
-
the melting temperature of the major allele holoenzyme is at 76.3°C Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
21.2
-
L-alanine major allele mutant enzyme F152A Homo sapiens
22.6
-
glyoxylate major allele wild mutant enzyme F152A Homo sapiens
33
-
L-alanine minor allele wild type enzyme Homo sapiens
33.6
-
L-alanine minor allele mutant enzyme F152I Homo sapiens
35
-
L-alanine major allele mutant enzyme F152I Homo sapiens
37
-
glyoxylate minor allele wild type enzyme Homo sapiens
39
-
glyoxylate major allele wild mutant enzyme F152I Homo sapiens
40
-
glyoxylate minor allele mutant enzyme F152I Homo sapiens
45
-
glyoxylate major allele wild type enzyme Homo sapiens
45
-
L-alanine major allele wild type enzyme Homo sapiens

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Homo sapiens

General Information

General Information Comment Organism
malfunction alanine:glyoxylate aminotransferase deficiency causes primary hyperoxaluria type 1 Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.46
-
L-alanine major allele mutant enzyme F152A Homo sapiens
0.82
-
L-alanine minor allele mutant enzyme F152I Homo sapiens
0.95
-
L-alanine major allele mutant enzyme F152I Homo sapiens
1.2
-
L-alanine minor allele wild type enzyme Homo sapiens
1.4
-
L-alanine major allele wild type enzyme Homo sapiens
66
-
glyoxylate major allele wild mutant enzyme F152A Homo sapiens
139
-
glyoxylate major allele wild mutant enzyme F152I Homo sapiens
160
-
glyoxylate minor allele mutant enzyme F152I Homo sapiens
168
-
glyoxylate minor allele wild type enzyme Homo sapiens
196
-
glyoxylate major allele wild type enzyme Homo sapiens