KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
glyoxylate | in 50 mM NaPO4 (pH 8.0), at 70°C | Hydrogenobacter thermophilus | |
8.1 | - |
L-alanine | in 50 mM NaPO4 (pH 8.0), at 70°C | Hydrogenobacter thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69000 | - |
gel filtration | Hydrogenobacter thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hydrogenobacter thermophilus | D2Z0I0 | - |
- |
Hydrogenobacter thermophilus TK-6 / IAM 12695 | D2Z0I0 | - |
- |
Purification (Comment) | Organism |
---|---|
butyl-Toyopearl column chromatography, DEAE-Toyopearl column chromatography, hydroxyapatite column chromatography, MonoQ column chromatography, and phenyl Superose gel filtration | Hydrogenobacter thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.071 | - |
enzyme from cell free extract | Hydrogenobacter thermophilus |
19 | - |
after 270fold purification | Hydrogenobacter thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + glyoxylate | high substrate specificity for Ala and glyoxylate | Hydrogenobacter thermophilus | pyruvate + glycine | - |
ir | |
L-alanine + glyoxylate | high substrate specificity for Ala and glyoxylate | Hydrogenobacter thermophilus TK-6 / IAM 12695 | pyruvate + glycine | - |
ir |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Hydrogenobacter thermophilus |
Synonyms | Comment | Organism |
---|---|---|
AGT | - |
Hydrogenobacter thermophilus |
alanine:glyoxylate aminotransferase | - |
Hydrogenobacter thermophilus |
aminotransferase 3 | - |
Hydrogenobacter thermophilus |
AT3 | - |
Hydrogenobacter thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | 8.4 | - |
Hydrogenobacter thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Hydrogenobacter thermophilus |