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Literature summary for 2.6.1.44 extracted from
- Novel archaeal alanine:glyoxylate aminotransferase from Thermococcus litoralis (2004), J. Bacteriol., 186, 5513-5518.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| hydroxylamine | 10 mM, complete inhibition | Thermococcus litoralis |  |
| L-Penicillamine | 10 mM, 86% inhibition | Thermococcus litoralis | |
| additional information | not inhibitory: 10 mM EDTA, 10 mM p-chloromercuribenzoate | Thermococcus litoralis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.32 | - |
glyoxylate | - |
Thermococcus litoralis | |
| 0.92 | - |
L-alanine | - |
Thermococcus litoralis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
4 * 42000, SDS-PAGE, 4 * 45169, claculated | Thermococcus litoralis |
| 45169 | - |
4 * 42000, SDS-PAGE, 4 * 45169, claculated | Thermococcus litoralis |
| 170000 | - |
PAGE | Thermococcus litoralis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermococcus litoralis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermococcus litoralis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 29 | - |
37°C, pH 7.5 | Thermococcus litoralis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-alanine + glyoxylate | - |
Thermococcus litoralis | glycine + pyruvate | - |
? | |
| additional information | strict specificity for L-alanine and glyoxylate as substrates | Thermococcus litoralis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | N-terminal animo acid sequence | Thermococcus litoralis |
| tetramer | 4 * 42000, SDS-PAGE, 4 * 45169, claculated | Thermococcus litoralis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity increases with temperature from 37°C to 90°C | Thermococcus litoralis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
10 min, stabel up to | Thermococcus litoralis |
| 90 | - |
10 min, less than 50% residual activity | Thermococcus litoralis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Thermococcus litoralis |
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Release 2023.1 (January 2023)
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