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Literature summary for 2.6.1.42 extracted from

  • Takpho, N.; Watanabe, D.; Takagi, H.
    Valine biosynthesis in Saccharomyces cerevisiae is regulated by the mitochondrial branched-chain amino acid aminotransferase Bat1 (2018), Microb. Cell, 5, 293-299 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.42

Protein Variants

Protein Variants Comment Organism
K202A site-directed mutagenesis, inactive mutant Saccharomyces cerevisiae
K219A site-directed mutagenesis, inactive mutant Saccharomyces cerevisiae
additional information construction of two artificial genes encoding the mitochondrial-targeting signal (MTS)-deleted Bat1 (Bat1-MTS) and the MTS of Bat1-fused Bat2 (Bat2+MTS) from originating Bat1 and Bat2 genes. Bat2+MTS is relocalized into the mitochondria, because Bat2 localization is changed from cytosol to the mitochondria by addition of MTS, and can partially restore the valine content and growth in DELTAbat1DELTAbat2 cells. Bat1-MTS and Bat2+MTS function properly in DELTAbat1DELTAbat2 cells. Mutant DELTAbat1DELTAbat2 cells harboring Bat1-MTS grow similarly to DELTAbat1 cells Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Saccharomyces cerevisiae 5829
-
mitochondrion
-
 Saccharomyces cerevisiae 5739
-
additional information subcellular localization of wild-type and mutant enzymes, overview Saccharomyces cerevisiae
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-isoleucine + 2-oxoglutarate Saccharomyces cerevisiae
-
 3-methyl-2-oxopentanoate + L-glutamate
-
 r
L-leucine + 2-oxoglutarate Saccharomyces cerevisiae
-
 4-methyl-2-oxopentanoate + L-glutamate
-
 r
L-valine + 2-oxoglutarate Saccharomyces cerevisiae
-
 3-methyl-2-oxobutanoate + L-glutamate
-
 r

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P38891
-
-
Saccharomyces cerevisiae P47176
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-isoleucine + 2-oxoglutarate
-
 Saccharomyces cerevisiae 3-methyl-2-oxopentanoate + L-glutamate
-
 r
L-leucine + 2-oxoglutarate
-
 Saccharomyces cerevisiae 4-methyl-2-oxopentanoate + L-glutamate
-
 r
L-valine + 2-oxoglutarate
-
 Saccharomyces cerevisiae 3-methyl-2-oxobutanoate + L-glutamate
-
 r

Subunits

Subunits Comment Organism
homodimer
-
 Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
Bat1
-
 Saccharomyces cerevisiae
BAT2
-
 Saccharomyces cerevisiae
BcaT
-
 Saccharomyces cerevisiae
branched-chain amino acid aminotransferase
-
 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate PLP Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction yeast strains with a single gene disruption of BAT1 or BAT2 are constructed and only DELTAbat1 cells show the slow-growth phenotype. There is no mitochondrial localization in mutant Bat1-MTS, whereas mutant Bat2+MTS is relocalized into the mitochondria. Bat1 and Bat2 isozymes deletion mutants phenotype analysis and comparison, detailed overview Saccharomyces cerevisiae
malfunction yeast strains with a single gene disruption of BAT1 or BAT2 are constructed and only DELTAbat1 cells show the slow-growth phenotype. There is no mitochondrial localization in mutant Bat1-MTS, whereas mutant Bat2+MTS is relocalized into the mitochondria. Bat1 and Bat2 isozymes deletion mutants phenotype analysis and comparison, detailed overview. The bat1 mutations affect valine but not leucine and isoleucine biosynthesis, lacking of Bat1 has the less effect on leucine biosynthesis Saccharomyces cerevisiae
metabolism the enzyme is involved in the branched-chain amino acid biosynthesis. The mitochondria are the major site of valine biosynthesis, and mitochondrial BCAT, Bat1, is important for valine biosynthesis in Saccharomyces cerevisiae. Unlike in higher eukaryotes, the Saccharomyces cerevisiae BCATs, Bat1 and Bat2, can function in both anabolic and catabolic pathways as the final step in the biosynthesis and the first step in the degradation of BCAAs Saccharomyces cerevisiae
physiological function isozymes Bat1 and Bat2 play distinct roles in branched-chain amino acid aminotransferase (BCAT) BCAAs biosynthesis Saccharomyces cerevisiae