Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-methyl-2-oxovalerate | enzyme TUZN1299 is inhibited by keto acceptors in substrate inhibition, the maximum activity toward 4-methyl-2-oxovalerate and 3-methyl-2-oxovalerate in the reaction with L-alanine is achieved at a concentration of keto acids 1 mM, at 20 mM the specific activity of the enzyme decreases by 60% and 80%, respectively | Thermoproteus uzoniensis | |
4-methyl-2-oxovalerate | enzyme TUZN1299 is inhibited by keto acceptors in substrate inhibition, the maximum activity toward 4-methyl-2-oxovalerate and 3-methyl-2-oxovalerate in the reaction with L-alanine is achieved at a concentration of keto acids 1 mM, at 20 mM the specific activity of the enzyme decreases by 60% and 80%, respectively | Thermoproteus uzoniensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | stopped-flow kinetics analysis of half-transamination reactions, steady-state kinetics | Thermoproteus uzoniensis | |
0.15 | - |
4-methyl-2-oxopentanoate | pH 8.0, 65°C | Thermoproteus uzoniensis | |
0.21 | - |
L-leucine | pH 8.0, 65°C | Thermoproteus uzoniensis | |
16 | - |
pyruvate | pH 8.0, 65°C | Thermoproteus uzoniensis | |
62 | - |
L-alanine | pH 8.0, 65°C | Thermoproteus uzoniensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + 2-oxobutyrate | Thermoproteus uzoniensis | - |
3-methyl-2-oxopentanoate + 2-aminobutyrate | - |
r | |
L-isoleucine + 2-oxobutyrate | Thermoproteus uzoniensis 768-20 | - |
3-methyl-2-oxopentanoate + 2-aminobutyrate | - |
r | |
L-leucine + 2-oxobutyrate | Thermoproteus uzoniensis | - |
4-methyl-2-oxopentanoate + 2-aminobutyrate | - |
r | |
L-valine + 2-oxobutyrate | Thermoproteus uzoniensis | - |
3-methyl-2-oxobutanoate + 2-aminobutyrate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoproteus uzoniensis | F2L0W0 | - |
- |
Thermoproteus uzoniensis 768-20 | F2L0W0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methyl-2-oxopentanoate + L-alanine | - |
Thermoproteus uzoniensis | L-leucine + pyruvate | - |
r | |
4-methyl-2-oxopentanoate + L-alanine | - |
Thermoproteus uzoniensis 768-20 | L-leucine + pyruvate | - |
r | |
L-glutamine + 2-oxobutyrate | - |
Thermoproteus uzoniensis | 2,5-dioxo-5-aminopentanoate + 2-aminobutyrate | - |
r | |
L-homoserine + 2-oxobutyrate | - |
Thermoproteus uzoniensis | ? + 2-aminobutyrate | - |
r | |
L-isoleucine + 2-oxobutyrate | - |
Thermoproteus uzoniensis | 3-methyl-2-oxopentanoate + 2-aminobutyrate | - |
r | |
L-isoleucine + 2-oxobutyrate | - |
Thermoproteus uzoniensis 768-20 | 3-methyl-2-oxopentanoate + 2-aminobutyrate | - |
r | |
L-leucine + 2-oxobutyrate | - |
Thermoproteus uzoniensis | 4-methyl-2-oxopentanoate + 2-aminobutyrate | - |
r | |
L-leucine + pyruvate | - |
Thermoproteus uzoniensis | 4-methyl-2-oxopentanoate + L-alanine | - |
r | |
L-leucine + pyruvate | - |
Thermoproteus uzoniensis 768-20 | 4-methyl-2-oxopentanoate + L-alanine | - |
r | |
L-methionine + 2-oxobutyrate | - |
Thermoproteus uzoniensis | 4-methylsulfanyl-2-oxobutanoate + 2-aminobutyrate | - |
r | |
L-methionine + 2-oxobutyrate | - |
Thermoproteus uzoniensis 768-20 | 4-methylsulfanyl-2-oxobutanoate + 2-aminobutyrate | - |
r | |
L-threonine + 2-oxobutyrate | - |
Thermoproteus uzoniensis | 2-oxo-3-hydroxybutyrate + 2-aminobutyrate | - |
r | |
L-valine + 2-oxobutyrate | - |
Thermoproteus uzoniensis | 3-methyl-2-oxobutanoate + 2-aminobutyrate | - |
r | |
additional information | enzyme TUZN1299 is highly active toward branched-chain amino acids (BCAAs), positively charged amino acids, L-methionine, L-threonine, L-homoserine, L-glutamine, as well as toward 2-oxobutyrate and keto analogues of BCAAs, whereas L-glutamate and 2-oxoglutarate are not converted in the overall reaction. The enzyme shows the highest specificity to BCAAs and their keto analogues. Glu188 forms a novel binding site for positively charged and polar side-chains of amino acids. Lack of accommodation for 2-oxoglutarate and L-glutamate is due to the unique orientation and chemical properties of residues 102-106 in the loop forming the A-pocket, molecular modelling and molecular mechanism, overview. The productive binding of 2-oxooglutarate and L-glutamate (both featuring negatively charged side-chains) in the active site of PMP-enzyme and PLP-enzyme models, respectively, is not observed | Thermoproteus uzoniensis | ? | - |
- |
|
additional information | enzyme TUZN1299 is highly active toward branched-chain amino acids (BCAAs), positively charged amino acids, L-methionine, L-threonine, L-homoserine, L-glutamine, as well as toward 2-oxobutyrate and keto analogues of BCAAs, whereas L-glutamate and 2-oxoglutarate are not converted in the overall reaction. The enzyme shows the highest specificity to BCAAs and their keto analogues. Glu188 forms a novel binding site for positively charged and polar side-chains of amino acids. Lack of accommodation for 2-oxoglutarate and L-glutamate is due to the unique orientation and chemical properties of residues 102-106 in the loop forming the A-pocket, molecular modelling and molecular mechanism, overview. The productive binding of 2-oxooglutarate and L-glutamate (both featuring negatively charged side-chains) in the active site of PMP-enzyme and PLP-enzyme models, respectively, is not observed | Thermoproteus uzoniensis 768-20 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
BcaT | - |
Thermoproteus uzoniensis |
branched-chain amino acid aminotransferase | - |
Thermoproteus uzoniensis |
TUZN1299 | - |
Thermoproteus uzoniensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
assay at | Thermoproteus uzoniensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.31 | - |
L-leucine | pH 8.0, 65°C | Thermoproteus uzoniensis | |
1.78 | - |
pyruvate | pH 8.0, 65°C | Thermoproteus uzoniensis | |
2.7 | - |
L-alanine | pH 8.0, 65°C | Thermoproteus uzoniensis | |
3 | - |
4-methyl-2-oxopentanoate | pH 8.0, 65°C | Thermoproteus uzoniensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Thermoproteus uzoniensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | the enzyme activity increases from pH 6.0 to pH 10.0 at 65°C | Thermoproteus uzoniensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | PLP, dependent on | Thermoproteus uzoniensis |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the PLP-dependent fold-type IV branched-chain amino acid aminotransferases (BCATs) from archaea, docking study and identification of subfamily-specific positions, overview | Thermoproteus uzoniensis |
additional information | molecular docking of 2-oxoglutarate, L-ornithine and L-glutamate into the molecular model of TUZN1299 | Thermoproteus uzoniensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.044 | - |
L-alanine | pH 8.0, 65°C | Thermoproteus uzoniensis | |
0.11 | - |
pyruvate | pH 8.0, 65°C | Thermoproteus uzoniensis | |
6.24 | - |
L-leucine | pH 8.0, 65°C | Thermoproteus uzoniensis | |
20 | - |
4-methyl-2-oxopentanoate | pH 8.0, 65°C | Thermoproteus uzoniensis |