Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method using 22-30% polyethlylene glycol 1500, 100 mM HEPES (pH 6.9-7.2), and 20 mM dithiothreitol | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C315A | contains changes in the structure of the beta-turn preceding the CXXC motif when compared with wild type protein, the oxidized mutant enzyme shows limited activity | Homo sapiens |
C315A/C318A | contains changes in the structure of the beta-turn preceding the CXXC motif when compared with wild type protein, the oxidized mutant enzyme shows limited activity | Homo sapiens |
C318A | contains changes in the structure of the beta-turn preceding the CXXC motif when compared with wild type protein, the oxidized mutant enzyme shows limited activity | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O15382 | - |
- |
Oxidation Stability | Organism |
---|---|
the activity of oxidized BCAT with 0.5 mM hydrogen peroxide for 2 h at 25°C shows 99.5% inhibition | Homo sapiens |
Purification (Comment) | Organism |
---|---|
Ni-NTA acid resin column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + 2-oxoglutarate | - |
Homo sapiens | 2-oxoglutarate + L-glutamate | - |
r | |
L-isoleucine + 2-oxoglutarate | - |
Homo sapiens | 3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | - |
Homo sapiens | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | - |
Homo sapiens | 3-methyl-2-oxobutanoate + L-glutamate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
BcaT | - |
Homo sapiens |
branched chain aminotransferase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
50.1 | - |
L-glutamate | mutant enzyme C315A/C318A | Homo sapiens | |
52 | - |
L-glutamate | mutant enzyme C315A | Homo sapiens | |
60.8 | - |
L-leucine | mutant enzyme C315A/C318A | Homo sapiens | |
65 | - |
L-leucine | mutant enzyme C315A | Homo sapiens | |
70.5 | - |
L-isoleucine | mutant enzyme C315A/C318A | Homo sapiens | |
72 | - |
L-isoleucine | mutant enzyme C315A | Homo sapiens | |
188 | - |
L-valine | mutant enzyme C315A | Homo sapiens | |
197 | - |
L-valine | mutant enzyme C315A/C318A | Homo sapiens | |
230 | - |
L-valine | mutant enzyme C318A | Homo sapiens | |
250 | - |
2-oxoglutarate | mutant enzyme C315A | Homo sapiens | |
265 | - |
L-glutamate | mutant enzyme C318A | Homo sapiens | |
270 | - |
2-oxoglutarate | mutant enzyme C315A/C318A | Homo sapiens | |
277 | - |
L-valine | wild type enzyme | Homo sapiens | |
290 | - |
L-glutamate | wild type enzyme | Homo sapiens | |
310 | - |
L-leucine | mutant enzyme C318A | Homo sapiens | |
319 | - |
L-isoleucine | mutant enzyme C318A | Homo sapiens | |
337 | - |
L-leucine | wild type enzyme | Homo sapiens | |
340 | - |
2-oxoglutarate | wild type enzyme | Homo sapiens | |
360 | - |
2-oxoglutarate | mutant enzyme C318A | Homo sapiens | |
371 | - |
L-isoleucine | wild type enzyme | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Homo sapiens |