Literature summary for 2.6.1.42 extracted from

Perez-Villasenor, G.; Tovar, A.R.; Moranchel, A.H.; Hernandez-Pando, R.; Hutson, S.M.; Torres, N.
Mitochondrial branched chain aminotransferase gene expression in AS-30D hepatoma rat cells and during liver regeneration after partial hepatectomy in rat (2005), Life Sci., 78, 334-339.

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1	-	L-leucine	mitochondria of AS-30D cell, pH 7.8, 37°C	Rattus norvegicus
1.3	-	L-isoleucine	mitochondria of AS-30D cell, pH 7.8, 37°C	Rattus norvegicus
2.1	-	L-valine	mitochondria of AS-30D cell, pH 7.8, 37°C	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
41000	-	x * 41000, SDS-PAGE	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	mitochondrial isoform	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
additional information	enzyme activity is associated with an immunoreactive band of 41000 Da, and an immunoreactive band of 43000 Da corresponds to inactive enzyme	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
AS-30D cell	hepatoma cell, expresses only mitochondrial isoform	Rattus norvegicus	-
liver	regenerating liver after partial hepatectomy, enzyme activity between day 3 and 6	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
7	-	regenerating liver, pH 7.8, 37°C	Rattus norvegicus
18.6	-	mitochondria of AS-30D cell, pH 7.8, 37°C	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-isoleucine + 2-oxoglutarate	-	Rattus norvegicus	3-methyl-2-oxopentanoate + L-glutamate	-	?
L-leucine + 2-oxoglutarate	-	Rattus norvegicus	2-oxoisohexanoate + L-glutamate	-	?
L-valine + 2-oxoglutarate	-	Rattus norvegicus	2-oxoisopentanoate + L-glutamate	-	?

Subunits

Subunits	Comment	Organism
?	x * 41000, SDS-PAGE	Rattus norvegicus

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Release 2023.1 (January 2023)