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Literature summary for 2.6.1.42 extracted from
- Mammalian branched-chain aminotransferases (2000), Methods Enzymol., 324, 355-365.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
| vector pET-28a, overexpression of recombinant BCATm and BCATc in Escherichia coli | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
| cytosol | - |
Rattus norvegicus | 5829 | - |
| mitochondrion | - |
Homo sapiens | 5739 | - |
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-isoleucine + 2-oxoglutarate | Homo sapiens | - |
3-methyl-2-oxopentanoate + L-glutamate | - |
r |  |
| L-isoleucine + 2-oxoglutarate | Rattus norvegicus | - |
3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
| L-leucine + 2-oxoglutarate | Homo sapiens | - |
4-methyl-2-oxopentanoate + L-glutamate | - |
? | |
| L-leucine + 2-oxoglutarate | Rattus norvegicus | - |
4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
| L-valine + 2-oxoglutarate | Rattus norvegicus | - |
3-methyl-2-oxobutanoate + L-glutamate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
human | - |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant Bcatm and BCATc, expressed in E. coli | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 85 | - |
recombinant cytosolic enzyme | Homo sapiens |
| 125 | - |
recombinant mitochondrial enzyme | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-isoleucine + 2-oxoglutarate | - |
Homo sapiens | 3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
| L-isoleucine + 2-oxoglutarate | - |
Rattus norvegicus | 3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
| L-leucine + 2-oxoglutarate | - |
Homo sapiens | 4-methyl-2-oxopentanoate + L-glutamate | - |
? | |
| L-leucine + 2-oxoglutarate | - |
Homo sapiens | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
| L-leucine + 2-oxoglutarate | - |
Rattus norvegicus | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
| L-valine + 2-oxoglutarate | - |
Homo sapiens | 3-methyl-2-oxobutanoate + L-glutamate | - |
r | |
| L-valine + 2-oxoglutarate | - |
Rattus norvegicus | 3-methyl-2-oxobutanoate + L-glutamate | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BCATc | - |
Homo sapiens |
| BCATc | - |
Rattus norvegicus |
| BCATm | - |
Homo sapiens |
| BCATm | - |
Rattus norvegicus |
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Release 2023.1 (January 2023)
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