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Literature summary for 2.6.1.42 extracted from

  • Xing, R.; Whitman, W.B.
    Characterization of amino acid aminotransferases of Methanococcus aeolicus (1974), J. Bacteriol., 174, 541-548.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate 5 mM 2-oxoglutarate inhibits the transamination of the 6.3-fold purified enzyme, of valine and isoleucine by about 50%, but the leucine activity is less affected. Inhibition is competitive with respect to valine Methanococcus aeolicus
L-cysteine 10 mM, inhibits activity with isoleucine and tyrosine by 22 and 34%, respectively Methanococcus aeolicus
L-isoleucine with 1 mM tyrosine as the substrate, 10 mM isoleucine inhibits activity by 94% Methanococcus aeolicus
L-leucine with 1 mM tyrosine as the substrate, 10 mM leucine inhibits activity by 93% Methanococcus aeolicus
L-methionine 10 mM methionine inhibits the activity with 1 mM tyrosine and phenylalanine by 86 and 59%, respectively, but it has no effect on the isoleucine activity Methanococcus aeolicus
L-phenylalanine with 2 mM isoleucine as the substrate, 10 mM phenylalanine inhibits activity by 46% Methanococcus aeolicus
L-valine with 1 mM tyrosine as the substrate, 10 mM valine inhibits activity by 78% Methanococcus aeolicus
additional information not inhibited by 5 mM KCI, NaCl, CuCl2, NiCl2, CoCl2, or FeCl2. No of activity with tyrosine by 10 mM aspartate, arginine, asparagine, glutamine, glycine, histidine, lysine, proline, serine, threonine or alanine Methanococcus aeolicus
ZnCl2 5 mM, 50% inhibition Methanococcus aeolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
L-phenylalanine pH and temperature not specified in the publication Methanococcus aeolicus
0.1
-
2-oxoglutarate pH and temperature not specified in the publication, cosubstrate: L-phenylalanine Methanococcus aeolicus
0.1
-
2-oxoglutarate pH and temperature not specified in the publication, cosubstrate: L-tryptophan Methanococcus aeolicus
0.1
-
2-oxoglutarate pH and temperature not specified in the publication, cosubstrate: L-tyrosine Methanococcus aeolicus
0.3
-
2-oxoglutarate pH and temperature not specified in the publication, cosubstrate: L-isoleucine Methanococcus aeolicus
0.3
-
2-oxoglutarate pH and temperature not specified in the publication, cosubstrate: L-valine Methanococcus aeolicus
0.6
-
2-oxoglutarate pH and temperature not specified in the publication, cosubstrate: L-leucine Methanococcus aeolicus
1.1
-
L-tyrosine pH and temperature not specified in the publication Methanococcus aeolicus
1.1
-
L-leucine pH and temperature not specified in the publication Methanococcus aeolicus
1.4
-
L-isoleucine pH and temperature not specified in the publication Methanococcus aeolicus
2.8
-
L-tryptophan pH and temperature not specified in the publication Methanococcus aeolicus
2.8
-
L-valine pH and temperature not specified in the publication Methanococcus aeolicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 5 mM MgCl2 stimulates by 26% Methanococcus aeolicus
Mn2+ 5 mM MgCl2 stimulates by 34% Methanococcus aeolicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
188000
-
gel filtration Methanococcus aeolicus

Organism

Organism UniProt Comment Textmining
Methanococcus aeolicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Methanococcus aeolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-isoleucine + 2-oxoglutarate
-
Methanococcus aeolicus L-glutamate + 3-methyl-2-oxopentanoate
-
?
L-leucine + 2-oxoglutarate
-
Methanococcus aeolicus 4-methyl-2-oxopentanoate + L-glutamate
-
?
L-phenylalanine + 2-oxoglutarate
-
Methanococcus aeolicus phenylpyruvate + L-glutamate
-
?
L-tryptophan + 2-oxoglutarate
-
Methanococcus aeolicus L-glutamic acid + 3-indole-2-oxopropanoate
-
?
L-tyrosine + 2-oxoglutarate
-
Methanococcus aeolicus p-hydroxyphenylpyruvate + L-glutamate
-
?
L-valine + 2-oxoglutarate
-
Methanococcus aeolicus 3-methyl-2-oxobutanoate + L-glutamate
-
?

Synonyms

Synonyms Comment Organism
BcaT
-
Methanococcus aeolicus
branched-chain aminotransferase
-
Methanococcus aeolicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Methanococcus aeolicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.9
-
2-oxoglutarate 6.3-fold purified enzyme, pH and temperature not specified in the publication Methanococcus aeolicus

pI Value

Organism Comment pI Value Maximum pI Value
Methanococcus aeolicus isoelectric focusing
-
4.9