BRENDA - Enzyme Database
show all sequences of 2.6.1.4

Isolation and characterization of a glutamate-glycine transaminase from human liver

Thompson, J.S.; Richardson, K.E.; Arch. Biochem. Biophys. 117, 599-603 (1966)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
strong
Homo sapiens
hydroxylamine
no inhibition
Homo sapiens
Isonicotinic acid hydrazide
no inhibition
Homo sapiens
Maleic acid
no inhibition
Homo sapiens
additional information
no inhibition by CN-
Homo sapiens
PCMB
-
Homo sapiens
phenylhydrazine
pyridoxal phosphate restores activity
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2
-
glutamate
pH 7.7, 37°C
Homo sapiens
2
-
glyoxylate
pH 7.7, 37°C
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glyoxylate + L-glutamate
Homo sapiens
may be involved in control of human oxalate synthesis by providing alternate pathway for glyoxylate metabolism
glycine + 2-oxoglutarate
-
Homo sapiens
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Purification (Commentary)
Commentary
Organism
290fold
Homo sapiens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.185
-
purified enzyme
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glyoxylate + L-alanine
-
639987
Homo sapiens
glycine + pyruvate
-
-
-
ir
glyoxylate + L-glutamate
best substrate
639987
Homo sapiens
glycine + 2-oxoglutarate
-
639987
Homo sapiens
ir
glyoxylate + L-glutamate
may be involved in control of human oxalate synthesis by providing alternate pathway for glyoxylate metabolism
639987
Homo sapiens
glycine + 2-oxoglutarate
-
639987
Homo sapiens
ir
glyoxylate + L-glutamine
-
639987
Homo sapiens
glycine + 2-oxoglutaramate
-
-
-
ir
additional information
substrate specificity
639987
Homo sapiens
?
-
-
-
-
additional information
poor substrates are arginine or methionine
639987
Homo sapiens
?
-
-
-
-
additional information
no activity with serine, valine, aspartate, histidine, phenylalanine, tyrosine, isoleucine
639987
Homo sapiens
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3
-
-
Homo sapiens
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.3
8.5
about half-maximal activity at pH 6.3 and about 60% of maximal activity at pH 8.5
Homo sapiens
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
addition to enzyme system mostly without effect; required; tightly bound to apoenzyme
Homo sapiens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
addition to enzyme system mostly without effect; required; tightly bound to apoenzyme
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
strong
Homo sapiens
hydroxylamine
no inhibition
Homo sapiens
Isonicotinic acid hydrazide
no inhibition
Homo sapiens
Maleic acid
no inhibition
Homo sapiens
additional information
no inhibition by CN-
Homo sapiens
PCMB
-
Homo sapiens
phenylhydrazine
pyridoxal phosphate restores activity
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2
-
glutamate
pH 7.7, 37°C
Homo sapiens
2
-
glyoxylate
pH 7.7, 37°C
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glyoxylate + L-glutamate
Homo sapiens
may be involved in control of human oxalate synthesis by providing alternate pathway for glyoxylate metabolism
glycine + 2-oxoglutarate
-
Homo sapiens
ir
Purification (Commentary) (protein specific)
Commentary
Organism
290fold
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.185
-
purified enzyme
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glyoxylate + L-alanine
-
639987
Homo sapiens
glycine + pyruvate
-
-
-
ir
glyoxylate + L-glutamate
best substrate
639987
Homo sapiens
glycine + 2-oxoglutarate
-
639987
Homo sapiens
ir
glyoxylate + L-glutamate
may be involved in control of human oxalate synthesis by providing alternate pathway for glyoxylate metabolism
639987
Homo sapiens
glycine + 2-oxoglutarate
-
639987
Homo sapiens
ir
glyoxylate + L-glutamine
-
639987
Homo sapiens
glycine + 2-oxoglutaramate
-
-
-
ir
additional information
substrate specificity
639987
Homo sapiens
?
-
-
-
-
additional information
poor substrates are arginine or methionine
639987
Homo sapiens
?
-
-
-
-
additional information
no activity with serine, valine, aspartate, histidine, phenylalanine, tyrosine, isoleucine
639987
Homo sapiens
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3
-
-
Homo sapiens
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.3
8.5
about half-maximal activity at pH 6.3 and about 60% of maximal activity at pH 8.5
Homo sapiens
Other publictions for EC 2.6.1.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738913
Zhang
Distinct photorespiratory reac ...
Oryza sativa Japonica Group
J. Photochem. Photobiol. B
142
110-117
2015
-
-
1
-
-
-
-
6
-
-
2
-
-
1
-
-
-
-
-
1
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-
4
1
-
-
-
3
-
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-
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1
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-
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-
6
-
-
2
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-
-
-
-
-
1
-
-
4
1
-
-
-
3
-
-
-
-
-
1
1
-
-
-
703680
Kameya
Purification of three aminotra ...
Hydrogenobacter thermophilus
FEBS J.
277
1876-1885
2010
-
-
-
-
-
-
-
2
-
-
2
-
-
4
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
1
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-
-
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-
-
-
2
-
-
2
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1
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1
-
-
-
-
-
-
1
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-
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-
-
-
731270
Donini
Recombinant production of eigh ...
Homo sapiens
Biochem. J.
422
265-272
2009
-
-
1
-
-
-
-
3
1
-
1
-
-
3
-
-
1
-
-
-
4
-
9
-
2
-
-
-
1
-
-
1
-
-
-
-
-
2
2
-
-
-
-
-
-
3
2
-
1
-
-
-
-
2
-
-
4
-
9
-
4
-
-
-
2
-
-
-
-
-
-
-
-
-
639991
Yamaguchi
Some properties of glycine ami ...
Rhodopseudomonas palustris, Rhodopseudomonas palustris No. 7
Biosci. Biotechnol. Biochem.
67
783-789
2003
-
-
-
-
-
-
4
2
-
-
2
2
-
2
-
-
1
-
-
1
1
1
4
1
1
-
1
2
1
1
1
1
-
-
-
-
-
-
1
-
-
-
-
4
-
2
-
-
2
2
-
-
-
1
-
1
1
1
4
1
1
-
1
2
1
1
1
-
-
-
-
-
-
-
636731
Orzechowski
Alanine aminotransferase and g ...
Zea mays
Acta Biochim. Pol.
46
447-457
1999
-
-
-
-
-
-
6
-
-
-
1
1
-
1
-
-
1
-
-
1
-
-
3
-
1
-
-
-
1
-
-
1
-
-
-
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-
-
1
-
-
-
-
6
-
-
-
-
1
1
-
-
-
1
-
1
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
639989
Galas
L-glutamate-glyoxylate aminotr ...
Lactobacillus plantarum
Acta Microbiol. Pol.
7
243-252
1975
-
-
-
-
-
-
-
2
-
1
1
-
-
1
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1
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1
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1
-
-
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1
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-
1
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-
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1
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-
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2
-
1
1
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1
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-
-
1
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1
-
-
-
1
-
-
-
-
-
-
-
-
-
639988
Rehfeld
Aminotransferases in peroxisom ...
Spinacia oleracea
J. Biol. Chem.
247
4803-4811
1972
-
-
-
-
-
-
1
2
2
-
-
1
-
1
-
-
-
-
-
1
1
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2
-
1
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-
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2
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-
1
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2
2
-
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1
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-
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-
-
1
1
-
2
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
639987
Thompson
-
Isolation and characterization ...
Homo sapiens
Arch. Biochem. Biophys.
117
599-603
1966
-
-
-
-
-
-
7
2
-
-
-
1
-
1
-
-
1
-
-
1
1
-
7
-
1
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
7
-
2
-
-
-
1
-
-
-
1
-
1
1
-
7
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
639986
Nakada
Glutamic-glycine transaminase ...
Rattus norvegicus
J. Biol. Chem.
239
468-471
1964
-
-
-
-
-
-
4
2
-
-
-
1
-
2
-
-
1
-
-
6
1
-
5
-
-
-
1
-
1
1
-
1
-
-
-
-
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-
1
-
-
-
-
4
-
2
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-
-
1
-
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1
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6
1
-
5
-
-
-
1
-
1
1
-
-
-
-
-
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-
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