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Literature summary for 2.6.1.4 extracted from
- Isolation and characterization of a glutamate-glycine transaminase from human liver (1966), Arch. Biochem. Biophys., 117, 599-603.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | strong | Homo sapiens |  |
| hydroxylamine | no inhibition | Homo sapiens | |
| Isonicotinic acid hydrazide | no inhibition | Homo sapiens | |
| Maleic acid | no inhibition | Homo sapiens | |
| additional information | no inhibition by CN- | Homo sapiens | |
| PCMB | - |
Homo sapiens | |
| phenylhydrazine | pyridoxal phosphate restores activity | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
glyoxylate | pH 7.7, 37°C | Homo sapiens | |
| 2 | - |
glutamate | pH 7.7, 37°C | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glyoxylate + L-glutamate | Homo sapiens | may be involved in control of human oxalate synthesis by providing alternate pathway for glyoxylate metabolism | glycine + 2-oxoglutarate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 290fold | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.185 | - |
purified enzyme | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glyoxylate + L-alanine | - |
Homo sapiens | glycine + pyruvate | - |
ir | |
| glyoxylate + L-glutamate | best substrate | Homo sapiens | glycine + 2-oxoglutarate | - |
ir | |
| glyoxylate + L-glutamate | may be involved in control of human oxalate synthesis by providing alternate pathway for glyoxylate metabolism | Homo sapiens | glycine + 2-oxoglutarate | - |
ir | |
| glyoxylate + L-glutamine | - |
Homo sapiens | glycine + 2-oxoglutaramate | - |
ir | |
| additional information | substrate specificity | Homo sapiens | ? | - |
? | |
| additional information | poor substrates are arginine or methionine | Homo sapiens | ? | - |
? | |
| additional information | no activity with serine, valine, aspartate, histidine, phenylalanine, tyrosine, isoleucine | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutamate-glycine transaminase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
- |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | 8.5 | about half-maximal activity at pH 6.3 and about 60% of maximal activity at pH 8.5 | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | required | Homo sapiens | |
| pyridoxal 5'-phosphate | tightly bound to apoenzyme | Homo sapiens | |
| pyridoxal 5'-phosphate | addition to enzyme system mostly without effect | Homo sapiens | |
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Release 2023.1 (January 2023)
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