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Literature summary for 2.6.1.4 extracted from
- Glutamic-glycine transaminase from rat liver (1964), J. Biol. Chem., 239, 468-471.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| hydroxylamine | 50% inhibition at 5 mM | Rattus norvegicus |  |
| Isonicotinic acid hydrazide | glyoxylate partially protects | Rattus norvegicus | |
| Maleic acid | weak | Rattus norvegicus | |
| PCMB | no inhibition | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.6 | - |
glutamate | pH 7.3 | Rattus norvegicus | |
| 8.3 | - |
glyoxylate | pH 7.3 | Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glyoxylate + L-glutamate | Rattus norvegicus | - |
glycine + 2-oxoglutarate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, about 30fold | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | limited activity | Rattus norvegicus | - |
| kidney | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
| additional information | tissue distribution, very low activity in lung, testes or diaphragm | Rattus norvegicus | - |
| spleen | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.13 | - |
partially purified enzyme | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glyoxylate + L-alanine | no activity | Rattus norvegicus | glycine + pyruvate | - |
? | |
| glyoxylate + L-glutamate | - |
Rattus norvegicus | glycine + 2-oxoglutarate | - |
ir | |
| additional information | substrate specificity | Rattus norvegicus | ? | - |
? | |
| additional information | no substrates are D-amino acid isomers | Rattus norvegicus | ? | - |
? | |
| additional information | no activity with ammonia, glutathione, L-ornithine or 4-aminobutanoate | Rattus norvegicus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutamic-glycine transaminase | - |
Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
5 min, stable | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
- |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | 8.2 | about half-maximal activity at pH 6.2 and pH 8.2 | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | addition to enzyme system mostly without effect | Rattus norvegicus | |
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Release 2023.1 (January 2023)
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