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Literature summary for 2.6.1.36 extracted from
- Synthesis of 1-piperideine-6-carboxylic acid produced by L-lysine-epsilon-aminotransferase from the Streptomyces clavuligerus gene expressed in Escherichia coli (2007), J. Agric. Food Chem., 55, 1767-1772.
Application
| Application | Comment | Organism |
|---|---|---|
| nutrition | enzyme is involved in generation of mousy off-flavors in wine. Enzymic product 2-aminoadipate 6-semialdehyde is further transformed to 1-piperideine-6-carboxylic acid, which may react with methylglyoxal to generate the acylated N-heterocyclic odorant 2-acetyltetrahydropyridine | Streptomyces clavuligerus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain JM109 | Streptomyces clavuligerus |
| expression in Escherichia coli | Streptomyces clavuligerus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48900 | - |
x * 49890, calculated, x * 48900, SDS-PAGE | Streptomyces clavuligerus |
| 49800 | - |
SDS-PAGE | Streptomyces clavuligerus |
| 49890 | - |
calculated from sequence of cDNA | Streptomyces clavuligerus |
| 49890 | - |
x * 49890, calculated, x * 48900, SDS-PAGE | Streptomyces clavuligerus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces clavuligerus | - |
- |
- |
| Streptomyces clavuligerus | Q5XPV2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Sephacryl S-200 gel filtration | Streptomyces clavuligerus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.12 | - |
pH 7.2, 37°C, wild-type enzyme | Streptomyces clavuligerus |
| 1.78 | - |
pH 7.2, 37°C, recombinant enzyme | Streptomyces clavuligerus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-lysine + 2-oxoglutarate | - |
Streptomyces clavuligerus | 2-aminoadipate 6-semialdehyde + ? | - |
? |  |
| L-lysine + 2-oxoglutarate | - |
Streptomyces clavuligerus | 2-aminoadipate 6-semialdehyde + 1-piperideine-6-carboxylic acid + L-glutamate | product 2-aminoadipate 6-semialdehyde is further transformed to 1-piperideine-6-carboxylic acid, which may react with methylglyoxal to generate the acylated N-heterocyclic odorant 2-acetyltetrahydropyridine | ? | |
| additional information | the enzyme is responsible for the formation of 1-piperideine-6-carboxylic acid, which may react with methylglyoxal to generate the acylated N-heterocyclic odorant 2-acetyltetrahydropyridine | Streptomyces clavuligerus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 49890, calculated, x * 48900, SDS-PAGE | Streptomyces clavuligerus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-lysine-epsilon-aminotransferase | - |
Streptomyces clavuligerus |
| LAT | - |
Streptomyces clavuligerus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Streptomyces clavuligerus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | - |
Streptomyces clavuligerus |
| 6.5 | 7.5 | broad | Streptomyces clavuligerus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | required | Streptomyces clavuligerus | |
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Release 2023.1 (January 2023)
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