Crystallization (Comment) | Organism |
---|---|
- |
[Flavobacterium] lutescens |
General Stability | Organism |
---|---|
pyridoxal 5'-phosphate, 0.1 mM, or 2-mercaptoethanol, 0.02%, protects against heat inactivation | [Flavobacterium] lutescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | incubation of the enzyme with L-lysine in the presence of high concentrations of phosphate gives an inactive form of the enzyme (semiapoenzyme), reactivation with pyridoxal 5'-phosphate | [Flavobacterium] lutescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
2-oxoglutarate | cosubstrate L-ornithine, pH 8.0, 37°C | [Flavobacterium] lutescens | |
0.5 | - |
2-oxoglutarate | L-lysine, pH 8.0, 37°C | [Flavobacterium] lutescens | |
2 | - |
L-ornithine | pH 8.0, 37°C | [Flavobacterium] lutescens | |
2.8 | - |
L-lysine | pH 8.0, 37°C | [Flavobacterium] lutescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
116000 | - |
sedimentation equilibrium method | [Flavobacterium] lutescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + 2-oxoglutarate | [Flavobacterium] lutescens | - |
2-aminoadipate 6-semialdehyde + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Flavobacterium] lutescens | - |
i.e. Achromobacter liquidum | - |
Purification (Comment) | Organism |
---|---|
- |
[Flavobacterium] lutescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18.67 | - |
pH 8.0, 37°C | [Flavobacterium] lutescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + 2-oxoglutarate | - |
[Flavobacterium] lutescens | 2-aminoadipate 6-semialdehyde + L-glutamate | - |
? | |
L-lysine + 2-oxoglutarate | 2-oxoglutarate is the only active amino acceptor | [Flavobacterium] lutescens | 2-aminoadipate 6-semialdehyde + L-glutamate | - |
? | |
L-ornithine + 2-oxoglutarate | - |
[Flavobacterium] lutescens | ? + L-glutamate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
pyridoxal 5'-phosphate, 0.1 mM, or 2-mercaptoethanol, 0.02%, protects against heat inactivation | [Flavobacterium] lutescens |
55 | - |
pH 6.0-7.5, 5 min, stable, partially purified enzyme | [Flavobacterium] lutescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
substrates L-ornithine + 2-oxoglutarate | [Flavobacterium] lutescens |
8.3 | 8.5 | substrates lysine + 2-oxoglutarate | [Flavobacterium] lutescens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | about 40% of maximum activity at pH 6 and pH 9.5, substrates ornithine + 2-oxoglutarate | [Flavobacterium] lutescens |
7 | 10 | pH 7: about 50% of maximum activity, pH 10: about 30% of maximum activity | [Flavobacterium] lutescens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | 55°C, 5 min, stable, partially purified enzyme | [Flavobacterium] lutescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | a pyridoxal phosphate protein | [Flavobacterium] lutescens | |
pyridoxal 5'-phosphate | 2 mol of pyridoxal 5'-phosphate per mol of holoenzyme | [Flavobacterium] lutescens | |
pyridoxal 5'-phosphate | Km: 0.000357 mM | [Flavobacterium] lutescens | |
pyridoxamine 5'-phosphate | can replace pyridoxal 5'-phosphate, Km: 0.00715 mM | [Flavobacterium] lutescens |