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Literature summary for 2.6.1.33 extracted from

  • Pandey, R.; Parajuli, P.; Chu, L.; Darsandhari, S.; Sohng, J.
    Biosynthesis of amino deoxy-sugar-conjugated flavonol glycosides by engineered Escherichia coli (2015), Biochem. Eng. J., 101, 191-199.
No PubMed abstract available

Application

Application Comment Organism
synthesis engineering of Escherichia coli to divert the flow of carbon flux from glucose-1-phosphate to thymidine diphosphate 4-keto 4,6-dideoxy-D-glucose (dTKDG), an intermediate of various dTDP-sugars. Glucose phosphate isomerase, glucose-6-phosphate dehydrogenase and uridylyltransferase genes are deleted while dTDP-D-glucose synthase and dTDP-Dglucose 4,6-dehydratase are overexpressed to produce a pool of dTKDG in the cell cytosol. The flow of dTKDG is further diverted to dTDP-D-viosamine, dTDP 4-amino 4,6-dideoxy-D-galactose, and dTDP 3-amino 3,6-dideoxy-D-galactose sugars using sugar aminotransferases gerB, wecE, and fdtB, respectively, from different sources. These sugar moieties are transferred to the 3-hydroxyl position of quercetin and kaempferol with the help of Arabidopsis thaliana glycosyltransferase ArGT3 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P27833
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Synonyms

Synonyms Comment Organism
WecE
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Escherichia coli