Cloned (Comment) | Organism |
---|---|
- |
Streptomyces venezuelae |
Crystallization (Comment) | Organism |
---|---|
hanging drop method of vapor diffusion. The structure of DesI is solved in complex with its product, dTDP-4-amino-4,6-dideoxyglucose, to a nominal resolution of 2.1 A. Each subunit of the dimeric enzyme contains 12 alpha-helices and 14 alpha-strands. Crystals belong to the space group P2(1)2(1)2(1) with unit cell dimensions of 59.5 A, 66.8 A, 242.0 A and one dimer per asymmetric unit. Crystal dimensions are typically 1 mm * 0.6 mm * 0.2 mm | Streptomyces venezuelae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose + L-glutamate | Streptomyces venezuelae | the enzyme is involved in biosynthesis of desosamine | dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose + 2-oxoglutarate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces venezuelae | Q9ZGH0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces venezuelae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose + L-glutamate | the enzyme is involved in biosynthesis of desosamine | Streptomyces venezuelae | dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose + 2-oxoglutarate | - |
? | |
dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose + L-glutamate | the two active sites of the enzyme are located in clefts at the subunit/subunit interface. Electron density corresponding to the bound product demonstrates a covalent bond between the amino group of the product and C-4' of the pyridoxal 5'-phosphate cofactor | Streptomyces venezuelae | dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose + 2-oxoglutarate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | each subunit of the dimeric enzyme contains 12 alpha-helices and 14 alpha-strands | Streptomyces venezuelae |
Synonyms | Comment | Organism |
---|---|---|
DesI | - |
Streptomyces venezuelae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Streptomyces venezuelae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Streptomyces venezuelae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | a pyridoxal-phosphate protein | Streptomyces venezuelae |