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Literature summary for 2.6.1.30 extracted from

  • Yoshikane, Y.; Yokochi, N.; Ohnishi, K.; Hayashi, H.; Yagi, T.
    Molecular cloning, expression and characterization of pyridoxamine-pyruvate aminotransferase (2006), Biochem. J., 396, 499-507.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.30

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strains JM109 and BL21(DE3) Mesorhizobium loti
expression in Escherichia coli Mesorhizobium loti

Protein Variants

Protein Variants Comment Organism
C198A enzyme shows the same specific activity as that of the wild type enzyme Mesorhizobium loti
C198A shows activity of the wild type enzyme Mesorhizobium loti
K197L mutant enzyme shows no activity Mesorhizobium loti
K197L shows no activity Mesorhizobium loti

Inhibitors

Inhibitors Comment Organism Structure
additional information hydroxylamine (10 mM), phenylhydrazine (1 mM) and sodium borohydride (1 mM) do not inactivate the enzyme Mesorhizobium loti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.044
-
 pyridoxamine
-
 Mesorhizobium loti
0.044
-
 pyridoxamine 30°C, pH 9.0 Mesorhizobium loti
0.059
-
 pyridoxal
-
 Mesorhizobium loti
0.059
-
 pyridoxal 30°C, pH 9.0 Mesorhizobium loti
0.34
-
 pyruvate
-
 Mesorhizobium loti
0.34
-
 pyruvate 30°C, pH 9.0 Mesorhizobium loti
7.1
-
 2-oxobutyrate
-
 Mesorhizobium loti
7.1
-
 2-oxobutyrate 30°C, pH 9.0 Mesorhizobium loti
11
-
 L-alanine
-
 Mesorhizobium loti
11
-
 L-alanine 30°C, pH 9.0 Mesorhizobium loti
20
-
 (S)-2-aminobutyrate
-
 Mesorhizobium loti
20
-
 (S)-2-aminobutyrate 30°C, pH 9.0 Mesorhizobium loti

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 4 * 41000, SDS-PAGE Mesorhizobium loti
41589
-
 4 * 41589, calculated from amino acid sequence Mesorhizobium loti
41589
-
 4 * 41589, calculated from sequence Mesorhizobium loti
154000
-
 gel filtration Mesorhizobium loti

Organism

Organism UniProt Comment Textmining
Mesorhizobium loti
-
-
-
Mesorhizobium loti Q988B8
-
-
Mesorhizobium loti MAFF303099
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Mesorhizobium loti
partial, QA52 column chromatography and butyl-Toyopearl column chromatography Mesorhizobium loti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2-aminobutyrate + pyruvate (S)-2-aminobutyrate is a poor substrate Mesorhizobium loti ?
-
 ?
(S)-2-aminobutyrate + pyruvate (S)-2-aminobutyrate is a poor substrate Mesorhizobium loti MAFF303099 ?
-
 ?
additional information the enzyme shows less than 0.5% of the activity of pyridoxamine towards pyridoxamine 5'-phosphate (3.3 mM) with pyruvate as the amino acceptor, the enzyme shows less than 0.5% of the activity of pyruvate towards 2-oxoglutarate, 3-hydroxypyruvate, indol-pyruvate, phenylpyruvate, 3-methyl-2-oxobutyrate and 4-hydroxyphenylpyruvate when they are used at 10 mM Mesorhizobium loti ?
-
 ?
additional information the enzyme shows less than 0.5% of the activity of pyridoxamine towards pyridoxamine 5'-phosphate (3.3 mM) with pyruvate as the amino acceptor, the enzyme shows less than 0.5% of the activity of pyruvate towards 2-oxoglutarate, 3-hydroxypyruvate, indol-pyruvate, phenylpyruvate, 3-methyl-2-oxobutyrate and 4-hydroxyphenylpyruvate when they are used at 10 mM Mesorhizobium loti MAFF303099 ?
-
 ?
pyridoxamine + 2-oxobutyrate
-
 Mesorhizobium loti ?
-
 ?
pyridoxamine + 2-oxobutyrate
-
 Mesorhizobium loti MAFF303099 ?
-
 ?
pyridoxamine + 2-oxobutyrate poor substrate Mesorhizobium loti pyridoxal + (S)-2-aminobutyrate
-
 r
pyridoxamine + pyruvate
-
 Mesorhizobium loti pyridoxal + L-alanine
-
 ?
pyridoxamine + pyruvate
-
 Mesorhizobium loti pyridoxal + L-alanine
-
 r
pyridoxamine + pyruvate
-
 Mesorhizobium loti MAFF303099 pyridoxal + L-alanine
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 41000, SDS-PAGE Mesorhizobium loti
homotetramer 4 * 41589, calculated from amino acid sequence Mesorhizobium loti
tetramer 4 * 41000, SDS-PAGE Mesorhizobium loti
tetramer 4 * 41589, calculated from sequence Mesorhizobium loti

Synonyms

Synonyms Comment Organism
PPAT
-
 Mesorhizobium loti
pyridoxamine-pyruvate aminotransferase
-
 Mesorhizobium loti

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 at pH 9, 100 mM borate/KOH buffer Mesorhizobium loti
70
-
 in 100 mM borate/KOH buffer pH 9.0 Mesorhizobium loti

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 80 30: 17% of maximal activity, 80°C: 3% of maximal activity Mesorhizobium loti

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 80 17% and 3% of the maximal activity is observed at 30°C and 80°C, respectively Mesorhizobium loti

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2 8 pyridoxamine
-
 Mesorhizobium loti
2 8 pyridoxamine 30°C, pH 9.0 Mesorhizobium loti
9
-
 (S)-2-aminobutyrate
-
 Mesorhizobium loti
9
-
 (S)-2-aminobutyrate 30°C, pH 9.0 Mesorhizobium loti
24
-
 2-oxobutyrate
-
 Mesorhizobium loti
24
-
 2-oxobutyrate 30°C, pH 9.0 Mesorhizobium loti
29
-
 pyruvate
-
 Mesorhizobium loti
29
-
 pyruvate 30°C, pH 9.0 Mesorhizobium loti
41
-
 L-alanine
-
 Mesorhizobium loti
41
-
 pyridoxal
-
 Mesorhizobium loti
41
-
 L-alanine 30°C, pH 9.0 Mesorhizobium loti
41
-
 pyridoxal 30°C, pH 9.0 Mesorhizobium loti

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
 estimated based on the Km and kcat values Mesorhizobium loti

Cofactor

Cofactor Comment Organism Structure
additional information pyridoxal 5'-phosphate-independent enzyme Mesorhizobium loti