Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | phosphate is neither essential nor inhibitory | Pseudomonas sp. |
Crystallization (Comment) | Organism |
---|---|
addition of saturated ammonium sulfate solution to the concentrated protein solution until the first permanent turbidity appears, several days at 5°C | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | phosphate is neither essential nor inhibitory | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
pyridoxamine | pH 8.5, 37°C | Pseudomonas sp. | |
0.1 | - |
pyruvate | pH 8.5, 37°C | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyridoxamine + pyruvate | Pseudomonas sp. | oxaloacetate and several other keto acids tested cannot replace pyruvate as amino group acceptor | pyridoxal + L-alanine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.3 | - |
purified enzyme | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyridoxamine + pyruvate | oxaloacetate and several other keto acids tested cannot replace pyruvate as amino group acceptor | Pseudomonas sp. | pyridoxal + L-alanine | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
10 min, stable | Pseudomonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | pyridoxal 5'-phosphate is not required for activity | Pseudomonas sp. |