Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-Methyltryptophan | can also act as substrate | Streptomyces griseus | |
p-chloromercuribenzoate | no inhibition | Streptomyces griseus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + 2-oxoglutarate | Streptomyces griseus | initial step of biosynthetic pathway of the antibiotic indolmycin | L-glutamate + 3-indole-2-oxopropanoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces griseus | - |
strain ATCC 12648 | - |
Purification (Comment) | Organism |
---|---|
partial, 3fold | Streptomyces griseus |
Storage Stability | Organism |
---|---|
-20°C, best stored by freezing the crude cell-free extract with 10% glycerol or by freezing the 45-60% ammonium sulfate precipitate in a phosphate buffer solution | Streptomyces griseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methyltryptophan + 2-oxoglutarate | can also inhibit the enzyme, stereospecific for positions 2 and 3 | Streptomyces griseus | 3-(3-methylindole)-2-oxopropanoate + L-glutamate | - |
? | |
L-phenylalanine + 2-oxoglutarate | - |
Streptomyces griseus | L-glutamate + phenylpyruvate | - |
? | |
L-tryptophan + 2-oxoglutarate | - |
Streptomyces griseus | L-glutamate + 3-indole-2-oxopropanoate | - |
? | |
L-tryptophan + 2-oxoglutarate | initial step of biosynthetic pathway of the antibiotic indolmycin | Streptomyces griseus | L-glutamate + 3-indole-2-oxopropanoate | - |
? | |
L-tyrosine + 2-oxoglutarate | - |
Streptomyces griseus | L-glutamate + 3-(4-hydroxyphenyl)-2-oxopropanoate | - |
? | |
additional information | no activity with D-tryptophan | Streptomyces griseus | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
10 | 12 h, complete loss of activity | Streptomyces griseus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Streptomyces griseus |