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Literature summary for 2.6.1.26 extracted from

  • Soffer, R.L.; Hechtman, P.; Savage, M.
    L-Triiodothyronine aminotransferase (1973), J. Biol. Chem., 248, 1224-1230.
    View publication on PubMed
Search for more literature for 2.6.1.26

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol absolutely required Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
p-hydroxymercuribenzoate complete inhibition at 0.1 mM Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.034
-
 L-triiodothyronine pH 7.8, 37°C Oryctolagus cuniculus
0.2
-
 2-oxoglutarate cosubstrate triiodothyronine, pH 7.8, 37°C Oryctolagus cuniculus
0.2
-
 2-oxoglutarate cosubstrate L-thyroxine, pH 7.8, 37°C Oryctolagus cuniculus
0.9
-
 3,5-diiodo-L-tyrosine pH 7.8, 37°C Oryctolagus cuniculus
2
-
 pyruvate cosubstrate L-triiodothyronine, pH 7.8, 37°C Oryctolagus cuniculus
2
-
 2-oxoglutarate cosubstrate 3,5-dinitro-L-tyrosine, pH 7.8, 37°C Oryctolagus cuniculus
2.12
-
 L-thyronine pH 7.8, 37°C Oryctolagus cuniculus
3.8
-
 3-iodo-L-tyrosine pH 7.8, 37°C Oryctolagus cuniculus
4.4
-
 3,5-Dinitro-L-tyrosine pH 7.8, 37°C Oryctolagus cuniculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Oryctolagus cuniculus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 2 * 48000, SDS-PAGE Oryctolagus cuniculus
95000
-
 glycerol density gradient centrifugation Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-3,5,3'-triiodothyronine + 2-oxoglutarate Oryctolagus cuniculus
-
 3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-glutamate i.e. 3,5,3'-triiodothyropyruvate + L-Glu r

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
brain low activity Oryctolagus cuniculus
-
heart low activity Oryctolagus cuniculus
-
kidney
-
 Oryctolagus cuniculus
-
liver
-
 Oryctolagus cuniculus
-
lung low activity Oryctolagus cuniculus
-
additional information not in serum Oryctolagus cuniculus
-
skeletal muscle low activity Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.013
-
 purified enzyme, substrates L-3,5,3'-triiodothyronine + 2-oxoglutarate Oryctolagus cuniculus
15
-
 purified enzyme, substrates 3,5-dinitro-L-tyrosine + 2-oxoglutarate Oryctolagus cuniculus

Storage Stability

Storage Stability Organism
-20°C, 30% loss of activity per month Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,5-dibromo-L-tyrosine + 2-oxoglutarate
-
 Oryctolagus cuniculus 3-(3,5-dibromo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
 r
3,5-diiodo-D-tyrosine + 2-oxoglutarate very low activity Oryctolagus cuniculus 3-(3,5-diiodo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
 r
3,5-diiodo-L-tyrosine + 2-oxoglutarate
-
 Oryctolagus cuniculus 3-(3,5-diiodo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
 r
3,5-dinitro-L-tyrosine + 2-oxoglutarate
-
 Oryctolagus cuniculus 3-(3,5-dinitro-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate i.e. 3,5-dinitro-p-hydroxyphenylpyruvic acid + Glu r
3-amino-L-tyrosine + 2-oxoglutarate very low activity Oryctolagus cuniculus 3-(3-amino-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
 r
3-iodo-L-tyrosine + 2-oxoglutarate
-
 Oryctolagus cuniculus 3-(3-iodo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
 r
3-nitro-L-tyrosine + 2-oxoglutarate
-
 Oryctolagus cuniculus 3-(3-nitro-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
 r
L-3,5,3'-triiodothyronine + 2-oxoglutarate
-
 Oryctolagus cuniculus 3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-glutamate i.e. 3,5,3'-triiodothyropyruvate + L-Glu r
L-3,5,3'-triiodothyronine + oxaloacetate
-
 Oryctolagus cuniculus 3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + glycine i.e. 3,5,3'-triiodothyrophenylpyruvate + Gly r
L-3,5,3'-triiodothyronine + pyruvate
-
 Oryctolagus cuniculus 3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-alanine i.e. 3,5,3'-triiodothyrophenylpyruvate + L-Ala r
L-phenylalanine + 2-oxoglutarate low activtiy Oryctolagus cuniculus 3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
 r
L-thyronine + 2-oxoglutarate
-
 Oryctolagus cuniculus ?
-
 ?
L-thyroxine + 2-oxoglutarate
-
 Oryctolagus cuniculus 3-[4-(4-hydroxy-3,5-diiodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-glutamate
-
 r
L-tryptophan + 2-oxoglutarate low activtiy Oryctolagus cuniculus 3-indole-2-oxopropanoate + L-glutamate
-
 r
L-tyrosine + 2-oxoglutarate low activtiy Oryctolagus cuniculus 2-oxo-3-phenylpropanoate + L-glutamate
-
 r
additional information specific for 2-oxoglutarate Oryctolagus cuniculus ?
-
 ?
additional information pyruvate and oxalacetate are ineffective with 3,5-dinitro-L-tyrosine and only partially effective with iodinated tyrosines Oryctolagus cuniculus ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 48000, SDS-PAGE Oryctolagus cuniculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0233
-
 L-thyroxine pH 7.8, 37°C Oryctolagus cuniculus
0.0317
-
 L-triiodothyronine pH 7.8, 37°C Oryctolagus cuniculus
1.1
-
 3,5-diiodo-L-tyrosine pH 7.8, 37°C Oryctolagus cuniculus
3.68
-
 3-iodo-L-tyrosine pH 7.8, 37°C Oryctolagus cuniculus
6.6
-
 3,5-diiodo-L-tyrosine pH 7.8, 37°C Oryctolagus cuniculus
39.9
-
 3,5-Dinitro-L-tyrosine pH 7.8, 37°C Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4 8.6 broad optimum Oryctolagus cuniculus

pH Range

pH Minimum pH Maximum Comment Organism
7 8.6 pH 7.0: 30% of maximal activity with L-triiodothyronine, 15% of maximal activity with 3,5-dinitro-L-tyrosine, pH 7.4-8.6: maximal activity with triiodothyronine and 3,5-dinitro-L-tyrosine Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate partial requirement Oryctolagus cuniculus