Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hydroxylamine | 70% inhibition at 1 mM | Rattus norvegicus | |
L-glutarate | 63% inhibition at 0.5 mM | Rattus norvegicus | |
Maleate | 65% inhibition at 5 mM | Rattus norvegicus | |
N-ethylmaleimide | 50% inhibition at 5 mM | Rattus norvegicus | |
p-chloromercuribenzoate | 77% inhibition at 0.5 mM | Rattus norvegicus | |
phenylhydrazine | 84% inhibition at 1 mM | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
2-oxoglutarate | pH 8.1, 37°C | Rattus norvegicus | |
33 | - |
L-glutamate | pH 8.1, 37°C | Rattus norvegicus | |
240 | - |
L-erythro-4-hydroxyglutamate | pH 8.1, 37°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxy-L-glutamate + oxo acid | Rattus norvegicus | metabolism of 4-hydroxy-L-glutamate in rat liver | 4-hydroxy-2-oxoglutarate + L-glutamate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.49 | - |
partially purified enzyme | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxy-L-glutamate + 2-oxoglutarate | enzyme utilises erythro-L-4-hydroxyglutamate and threo-L-4-hydroxyglutamate to the same extent, but no D-isomers | Rattus norvegicus | 4-hydroxy-2-oxoglutarate + L-glutamate | - |
r | |
4-hydroxy-L-glutamate + oxaloacetate | - |
Rattus norvegicus | 4-hydroxy-2-oxoglutarate + L-aspartate | - |
r | |
4-hydroxy-L-glutamate + oxo acid | metabolism of 4-hydroxy-L-glutamate in rat liver | Rattus norvegicus | 4-hydroxy-2-oxoglutarate + L-glutamate | - |
r | |
L-erythro-4-hydroxyglutamate + 2-oxoglutarate | - |
Rattus norvegicus | D-4-hydroxy-2-oxoglutarate + L-glutamate | - |
? | |
additional information | substrate stereospecificity | Rattus norvegicus | ? | - |
? | |
additional information | pyruvate is a poor substrate | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | may be identical with EC 2.6.1.1 aspartate transaminase | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
with erythro-L-hydroxyglutamic acid | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.7 | 9.5 | pH 6.7: about 45% of activity maximum, pH 9.5: about 55% of activity maximum | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor requirement | Rattus norvegicus |