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Literature summary for 2.6.1.22 extracted from

  • Tamaki, N.; Sakata, S.F.; Matsuda, K.
    Purification, properties, and sequencing of aminoisobutyrate aminotransferases from rat liver (2000), Methods Enzymol., 324, 376-389.
    View publication on PubMed
Search for more literature for 2.6.1.22

Inhibitors

Inhibitors Comment Organism Structure
5-fluorouracil
-
 Rattus norvegicus
5-Iodouracil
-
 Rattus norvegicus
6-Azathymine
-
 Rattus norvegicus
6-Azauracil
-
 Rattus norvegicus
gabaculine
-
 Rattus norvegicus
additional information not inhibitory: 6-azauridine, 6-azauridine 5’-phosphate Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
 beta-Alanine pH 8.8, 37°C Rattus norvegicus
2.7
-
 (S)-3-amino-2-methylpropanoate pH 8.8, 37°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
105000
-
 gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.047
-
 kidney enzyme, pH 8.8, 37°C Rattus norvegicus
0.096
-
 brain enzyme, pH 8.8, 37°C Rattus norvegicus
0.133
-
 liver enzyme, pH 8.8, 37°C Rattus norvegicus

Storage Stability

Storage Stability Organism
-25°C, in 0.1 M phosphate buffer, pH 7.0, 1mM EDTA, 2 mM 2-mercaptoethanol, 0.04 mM pyridoxal-5'-phosphate, few days, 50% loss of activity Rattus norvegicus
4°C, in 0.1 M phosphate buffer, pH 7.0, 1mM EDTA, 2 mM 2-mercaptoethanol, 0.04 mM pyridoxal-5'-phosphate, 1 week, no loss of activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-3-amino-2-methylpropanoate + pyruvate
-
 Rattus norvegicus D-methylmalonate semialdehyde + L-alanine
-
 r
4-aminobutanoate + 2-oxoglutarate transamination at 150% the rate of (S)-3-amino-2-methylpropanoate Rattus norvegicus succinic semialdehyde + L-glutamate
-
 r
5-aminopentanoate + 2-oxoglutarate transamination at 145% the rate of (S)-3-amino-2-methylpropanoate Rattus norvegicus 5-oxopentanoate + L-glutamate
-
 r
beta-alanine + 2-oxoglutarate as good as (S)-3-amino-2-methylpropanoate Rattus norvegicus malonic semialdehyde + L-glutamate
-
 r
additional information no substrates are glyoxylate, pyruvate, oxalacetate, phenylpyruvate Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 x 56000, SDS-PAGE Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.2
-
-
 Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0071
-
 gabaculine pH 8.8, 37°C Rattus norvegicus
0.7
-
 6-Azauracil pH 8.8, 37°C Rattus norvegicus
1.8
-
 5-fluorouracil uncompetitive against 2-oxoglutarate, pH 8.8, 37°C Rattus norvegicus
1.9
-
 5-fluorouracil competitive to beta-alanine, pH 8.8, 37°C Rattus norvegicus