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Literature summary for 2.6.1.21 extracted from

  • Ro, H.S.; Hong, S.P.; Seo, H.J.; Yoshimura, T.; Esaki, N.; Soda, K.; Kim, H.S.; Sung, M.H.
    Site-directed mutagenesis of the amino acid residues in beta-strand III [Val30-Val36] of D-amino acid aminotransferase of Bacillus sp. YM-1 (1996), FEBS Lett., 398, 141-145.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Bacillus sp. (in: Bacteria)

Protein Variants

Protein Variants Comment Organism
E32A 1% of wild-type activity Bacillus sp. (in: Bacteria)
E32D 65% of wild-type activity Bacillus sp. (in: Bacteria)
E32Q 10% of wild-type activity Bacillus sp. (in: Bacteria)
K33A 64% of wild-type activity Bacillus sp. (in: Bacteria)
V33A 110% of wild-type activity Bacillus sp. (in: Bacteria)
Y31A 0.4% of wild-type activity Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2
-
D-alanine pH 8.5, 50°C, E32D mtant enzyme Bacillus sp. (in: Bacteria)
1.4
-
2-oxoglutarate pH 8.5, 50°C, E32Q mutant enzyme Bacillus sp. (in: Bacteria)
1.8
-
2-oxoglutarate pH 8.5, 50°C, E32A mutant enzyme Bacillus sp. (in: Bacteria)
2.1
-
D-alanine pH 8.5, 50°C, wild-type and E32Q mutant enzyme Bacillus sp. (in: Bacteria)
2.1
-
2-oxoglutarate pH 8.5, 50°C, E32D mutant enzyme Bacillus sp. (in: Bacteria)
2.4
-
2-oxoglutarate pH 8.5, 50°C, Y33A mutant enzyme Bacillus sp. (in: Bacteria)
2.5
-
2-oxoglutarate pH 8.5, 50°C, wild-type enzyme Bacillus sp. (in: Bacteria)
10.1
-
2-oxoglutarate pH 8.5, 50°C, Y31A mutant enzyme Bacillus sp. (in: Bacteria)
15.5
-
2-oxoisovalerate pH 8.5, 50°C, V33A mutant enzyme Bacillus sp. (in: Bacteria)
28
-
2-oxoisovalerate pH 8.5, 50°C, wild-type enzyme Bacillus sp. (in: Bacteria)
32.2
-
2-oxoisovalerate pH 8.5, 50°C, K35A mutant enzyme Bacillus sp. (in: Bacteria)
35.6
-
2-oxoglutarate pH 8.5, 50°C, K35A mutant enzyme Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes, Resource Q, phenyl-superose Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-alanine + 2-oxoisovalerate
-
Bacillus sp. (in: Bacteria) pyruvate + D-valine
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.5
-
D-alanine pH 8.5, 50°C, Y31A mutant enzyme Bacillus sp. (in: Bacteria)
1.3
-
D-alanine pH 8.5, 50°C, E32A mutant enzyme Bacillus sp. (in: Bacteria)
13.4
-
D-alanine pH 8.5, 50°C, E32Q mutant enzyme Bacillus sp. (in: Bacteria)
84.3
-
D-alanine pH 8.5, 50°C, K35A mutant enzyme Bacillus sp. (in: Bacteria)
85.6
-
D-alanine pH 8.5, 50°C, E32D mutant enzyme Bacillus sp. (in: Bacteria)
131
-
D-alanine pH 8.5, 50°C, wild-type enzyme Bacillus sp. (in: Bacteria)
145
-
D-alanine pH 8.5, 50°C, V33A mutant enzyme Bacillus sp. (in: Bacteria)