Cloned (Comment) | Organism |
---|---|
expression of wild-type, L201A and L201W mutant enzymes in Escherichia coli | Bacillus sp. (in: Bacteria) |
Protein Variants | Comment | Organism |
---|---|---|
L201A | 2% of wild-type kcat | Bacillus sp. (in: Bacteria) |
L201W | 0.043% of wild-type kcat | Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | L201A and L201W mutant enzymes lose their activity by incubation with D-alanine with biphasic kinetics | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.63 | - |
2-oxoglutarate | pH 8.0, 37°C, wild-type enzyme | Bacillus sp. (in: Bacteria) | |
0.67 | - |
2-oxoglutarate | pH 8.0, 37°C, L201W mutant enzyme | Bacillus sp. (in: Bacteria) | |
1.2 | - |
D-alanine | pH 8.0, 37°C, L201W mutant enzyme | Bacillus sp. (in: Bacteria) | |
1.5 | - |
D-alanine | pH 8.0, 37°C, L201A mutant enzyme | Bacillus sp. (in: Bacteria) | |
4.8 | - |
2-oxoglutarate | pH 8.0, 37°C, L201A mutant enzyme | Bacillus sp. (in: Bacteria) | |
20 | - |
D-alanine | pH 8.0, 37°C, wild-type enzyme | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
YM-1, thermophile | - |
Bacillus sp. (in: Bacteria) YM-1 | - |
YM-1, thermophile | - |
Purification (Comment) | Organism |
---|---|
wild-type, L201A and L201W mutant enzyme, DEAE-toyopearl | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-alanine + 2-oxoglutarate | - |
Bacillus sp. (in: Bacteria) | pyruvate + D-glutamate | - |
r | |
D-alanine + 2-oxoglutarate | - |
Bacillus sp. (in: Bacteria) YM-1 | pyruvate + D-glutamate | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
D-alanine | pH 8.0, 37°C, L201W mutant enzyme | Bacillus sp. (in: Bacteria) | |
5.7 | - |
D-alanine | pH 8.0, 37°C, L201A mutant enzyme | Bacillus sp. (in: Bacteria) | |
280 | - |
D-alanine | pH 8.0, 37°C, wild-type enzyme | Bacillus sp. (in: Bacteria) |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | wild-type, L201A and L201W mutant enzyme: 0.89, 0.78 and 0.56 mol per mol of subunit, respectively | Bacillus sp. (in: Bacteria) |