Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.6.1.2 extracted from

  • Rojas-Ortega, E.; Aguirre-Lopez, B.; Reyes-Vivas, H.; Gonzalez-Andrade, M.; Campero-Basaldua, J.; Pardo, J.; Gonzalez, A.
    Saccharomyces cerevisiae differential functionalization of presumed ScALT1 and ScALT2 alanine transaminases has been driven by diversification of pyridoxal phosphate interactions (2018), Front. Microbiol., 9, 944 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta2 (DE3) cells Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.67
-
 2-oxoglutarate at pH 7.5 and 25°C Saccharomyces cerevisiae
10.39
-
 L-alanine at pH 7.5 and 25°C Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
147000
-
 gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-alanine + 2-oxoglutarate Saccharomyces cerevisiae
-
 pyruvate + L-glutamate
-
 r

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine + 2-oxoglutarate
-
 Saccharomyces cerevisiae pyruvate + L-glutamate
-
 r

Subunits

Subunits Comment Organism
homodimer 2 * 61000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
ALT1
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
698
-
 L-alanine at pH 7.5 and 25°C Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Saccharomyces cerevisiae