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Literature summary for 2.6.1.2 extracted from

  • Escalera-Fanjul, X.; Campero-Basaldua, C.; Colon, M.; Gonzalez, J.; Marquez, D.; Gonzalez, A.
    Evolutionary diversification of alanine transaminases in yeast Catabolic specialization and biosynthetic redundancy (2017), Front. Microbiol., 8, 1150 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta2 (DE3) cells Kluyveromyces lactis
expressed in Escherichia coli Rosetta2 (DE3) cells Lachancea kluyveri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
 2-oxoglutarate pH and temperature not specified in the publication Lachancea kluyveri
0.92
-
 2-oxoglutarate pH and temperature not specified in the publication Kluyveromyces lactis
4.88
-
 L-alanine pH and temperature not specified in the publication Lachancea kluyveri
17.25
-
 L-alanine pH and temperature not specified in the publication Kluyveromyces lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100100
-
 dynamic light scattering Lachancea kluyveri
135900
-
 dynamic light scattering Kluyveromyces lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-alanine + 2-oxoglutarate Saccharomyces cerevisiae
-
 pyruvate + L-glutamate
-
 ?
L-alanine + 2-oxoglutarate Kluyveromyces lactis
-
 pyruvate + L-glutamate
-
 ?
L-alanine + 2-oxoglutarate Lachancea kluyveri
-
 pyruvate + L-glutamate
-
 ?
L-alanine + 2-oxoglutarate Kluyveromyces lactis 155-2
-
 pyruvate + L-glutamate
-
 ?
L-alanine + 2-oxoglutarate Lachancea kluyveri Y156-2
-
 pyruvate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Kluyveromyces lactis
-
-
-
Kluyveromyces lactis 155-2
-
-
-
Lachancea kluyveri
-
-
-
Lachancea kluyveri Y156-2
-
-
-
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Kluyveromyces lactis
Ni-NTA agarose column chromatography Lachancea kluyveri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine + 2-oxoglutarate
-
 Saccharomyces cerevisiae pyruvate + L-glutamate
-
 ?
L-alanine + 2-oxoglutarate
-
 Kluyveromyces lactis pyruvate + L-glutamate
-
 ?
L-alanine + 2-oxoglutarate
-
 Lachancea kluyveri pyruvate + L-glutamate
-
 ?
L-alanine + 2-oxoglutarate
-
 Kluyveromyces lactis 155-2 pyruvate + L-glutamate
-
 ?
L-alanine + 2-oxoglutarate
-
 Lachancea kluyveri Y156-2 pyruvate + L-glutamate
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 50000, SDS-PAGE Kluyveromyces lactis
homodimer 2 * 50000, SDS-PAGE Lachancea kluyveri
homodimer 2 * 59230, calculated from amino acid sequence Kluyveromyces lactis
homodimer 2 * 61160, calculated from amino acid sequence Lachancea kluyveri

Synonyms

Synonyms Comment Organism
ALT1
-
 Saccharomyces cerevisiae
ALT1
-
 Kluyveromyces lactis
ALT1
-
 Lachancea kluyveri

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
203
-
 L-alanine pH and temperature not specified in the publication Kluyveromyces lactis
355
-
 L-alanine pH and temperature not specified in the publication Lachancea kluyveri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Kluyveromyces lactis
7
-
-
 Lachancea kluyveri

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Saccharomyces cerevisiae
pyridoxal 5'-phosphate
-
 Kluyveromyces lactis
pyridoxal 5'-phosphate
-
 Lachancea kluyveri