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Literature summary for 2.6.1.2 extracted from

  • Ward, D.E.; Kengen, S.W.; van Der Oost, J.; de Vos, W.M.
    Purification and characterization of the alanine aminotransferase from the hyperthermophilic Archaeon pyrococcus furiosus and its role in alanine production (2000), J. Bacteriol., 182, 2559-2566.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.9
-
2-oxoglutarate pH 7.2, 80°C, native enzyme Pyrococcus furiosus
1.1
-
2-oxoglutarate pH 7.2, 80°C, recombinant enzyme Pyrococcus furiosus
2.8
-
alanine pH 7.2, 80°C, native enzyme Pyrococcus furiosus
3.2
-
L-alanine pH 7.2, 80°C, recombinant enzyme Pyrococcus furiosus
4.3
-
glutamate pH 7.2, 80°C, native enzyme Pyrococcus furiosus
4.7
-
glutamate pH 7.2, 80°C, recombinant enzyme Pyrococcus furiosus
5.4
-
pyruvate pH 7.2, 80°C, native enzyme Pyrococcus furiosus
5.6
-
pyruvate pH 7.2, 80°C, recombinant enzyme Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
2 * 46000, SDS-PAGE Pyrococcus furiosus
93400
-
gel filtration Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q9P9M8
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme, Q-Sepharose, phenyl-Sepharose, hydroxyapatite, S-Sepharose, Mono Q, Superdex 200, recombinant enzyme, heat treatment, Mono Q Pyrococcus furiosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
158
-
native enzyme Pyrococcus furiosus
243
-
recombinant enzyme Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aspartate + 2-oxoglutarate 14% of activity with glutamate and pyruvate Pyrococcus furiosus malate + L-glutamate
-
?
aspartate + pyruvate 10% of activity with alanine and 2-oxoglutarate Pyrococcus furiosus malate + alanine
-
?
L-alanine + 2-oxoglutarate
-
Pyrococcus furiosus pyruvate + L-glutamate
-
r

Subunits

Subunits Comment Organism
dimer 2 * 46000, SDS-PAGE Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
above 95°C, native and recombinant enzyme Pyrococcus furiosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 95 native and recombinant enzyme Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.8 native and recombinant enzyme Pyrococcus furiosus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.2 native and recombinant enzyme Pyrococcus furiosus