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Literature summary for 2.6.1.2 extracted from
- Separation and properties of leaf aspartate aminotransferase and alanine aminotransferase isoenzymes operative in the C4 pathway of photosynthesis (1973), Arch. Biochem. Biophys., 156, 207-214.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| malate | 5 mM, 25% inhibition | Atriplex spongiosa |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.022 | - |
pyruvate | pH 7.5, 22°C, isoenzyme 3 | Atriplex spongiosa | |
| 0.028 | - |
2-oxoglutarate | pH 7.5, 22°C, isoenzyme 3 | Atriplex spongiosa | |
| 0.04 | - |
pyruvate | pH 7.5, 22°C, isoenzyme 2 | Atriplex spongiosa | |
| 0.09 | - |
2-oxoglutarate | pH 7.5, 22°C, isoenzyme 2 | Atriplex spongiosa | |
| 0.25 | - |
alanine | pH 7.5, 22°C, isoenzyme 1 | Atriplex spongiosa | |
| 0.3 | - |
2-oxoglutarate | pH 7.5, 22°C, isoenzyme 2 | Atriplex spongiosa | |
| 0.45 | - |
pyruvate | pH 7.5, 22°C, isoenzyme 1 | Atriplex spongiosa | |
| 0.55 | - |
glutamate | pH 7.5, 22°C, isoenzyme 1 | Atriplex spongiosa | |
| 0.8 | - |
glutamate | pH 7.5, 22°C, isoenzyme 3 | Atriplex spongiosa | |
| 1.15 | - |
glutamate | pH 7.5, 22°C, isoenzyme 2 | Atriplex spongiosa | |
| 3 | - |
alanine | pH 7.5, 22°C, isoenzyme 2 | Atriplex spongiosa | |
| 3.1 | - |
alanine | pH 7.5, 22°C, isoenzyme 3 | Atriplex spongiosa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Atriplex spongiosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, DEAE-cellulose, 3 isoforms | Atriplex spongiosa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Atriplex spongiosa | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, stable for several h in solution | Atriplex spongiosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-alanine + 2-oxoglutarate | - |
Atriplex spongiosa | pyruvate + L-glutamate | - |
r | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
isoenzymes 2 and 3, 10-20% loss of activity after 3 h | Atriplex spongiosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Atriplex spongiosa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | 8.7 | at least 50% of maximal activity at pH 6.4 and 8.7 | Atriplex spongiosa |
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