Literature summary for 2.6.1.19 extracted from

Trobacher, C.; Clark, S.; Bozzo, G.; Mullen, R.; DeEll, J.; Shelp, B.
Catabolism of GABA in apple fruit: Subcellular localization and biochemical characterization of two gamma-aminobutyrate transaminases (2013), Postharvest Biol. Technol., 75, 106-113.

No PubMed abstract available

Search for more literature for 2.6.1.19

Cloned(Commentary)

Cloned (Comment)	Organism
removal of the N-terminal targeting presequences yields good recovery of the soluble recombinant proteins in Escherichia coli when they are co-expressed with the GroES/EL molecular chaperone complex	Malus domestica

Inhibitors

Inhibitors	Comment	Organism	Structure
5-amino-1,3-cyclohexadienylcarboxylate	-	Malus domestica
Aminooxyacetate	-	Malus domestica
beta-Alanine	moderate inhibitor; moderate inhibitor	Malus domestica
ornithine	moderate inhibitor; moderate inhibitor	Malus domestica

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0165	-	glyoxylate	pH 9, 25°C, fixed substrate: 4-aminobutanoate	Malus domestica
0.0188	-	pyruvate	pH 9, 25°C, fixed substrate: 4-aminobutanoate	Malus domestica
0.023	-	pyruvate	pH 9, 25°C, fixed substrate: 4-aminobutanoate	Malus domestica
0.039	-	glyoxylate	pH 9, 25°C, fixed substrate: 4-aminobutanoate	Malus domestica
0.077	-	4-aminobutanoate	pH 9, 25°C, fixed substrate: pyruvate	Malus domestica
0.099	-	4-aminobutanoate	pH 9, 25°C, fixed substrate: glyoxylate	Malus domestica
0.135	-	4-aminobutanoate	pH 9, 25°C, fixed substrate: glyoxylate	Malus domestica
0.165	-	4-aminobutanoate	pH 9, 25°C, fixed substrate: pyruvate	Malus domestica

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Malus domestica	5739	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
54200	-	SDS-PAGE, single recombinant truncated MdGABA-T	Malus domestica

Organism

Organism	UniProt	Comment	Textmining
Malus domestica	J9XGP8	-	-
Malus domestica	J9XGZ5	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-aminobutanoate + glyoxylate	-	Malus domestica	4-oxobutanoate + glycine	-	?
4-aminobutanoate + pyruvate	-	Malus domestica	4-oxobutanoate + L-alanine	-	?

Synonyms

Synonyms	Comment	Organism
GABA-T	-	Malus domestica
gamma-aminobutyrate transaminase	-	Malus domestica
MdGABA-T1	-	Malus domestica
MdGABA-T2	-	Malus domestica

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Malus domestica

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.74	-	glyoxylate	pH 9, 25°C, fixed substrate: 4-aminobutanoate	Malus domestica
0.8	-	pyruvate	pH 9, 25°C, fixed substrate: 4-aminobutanoate	Malus domestica
0.84	-	4-aminobutanoate	pH 9, 25°C, fixed substrate: pyruvate	Malus domestica
1	-	4-aminobutanoate	pH 9, 25°C, fixed substrate: glyoxylate	Malus domestica
2.6	-	glyoxylate	pH 9, 25°C, fixed substrate: 4-aminobutanoate	Malus domestica
2.6	-	4-aminobutanoate	pH 9, 25°C, fixed substrate: pyruvate	Malus domestica
2.7	-	pyruvate	pH 9, 25°C, fixed substrate: 4-aminobutanoate	Malus domestica
2.7	-	4-aminobutanoate	pH 9, 25°C, fixed substrate: glyoxylate	Malus domestica

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	assay at	Malus domestica

pH Range

pH Minimum	pH Maximum	Comment	Organism
8.5	9.5	maximal activity	Malus domestica

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Malus domestica

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
10.4	-	4-aminobutanoate	pH 9, 25°C, fixed substrate: glyoxylate	Malus domestica
10.9	-	4-aminobutanoate	pH 9, 25°C, fixed substrate: pyruvate	Malus domestica
16.1	-	4-aminobutanoate	pH 9, 25°C, fixed substrate: pyruvate	Malus domestica
19.5	-	4-aminobutanoate	pH 9, 25°C, fixed substrate: glyoxylate	Malus domestica
42.7	-	pyruvate	pH 9, 25°C, fixed substrate: 4-aminobutanoate	Malus domestica
45.7	-	glyoxylate	pH 9, 25°C, fixed substrate: 4-aminobutanoate	Malus domestica
66.6	-	glyoxylate	pH 9, 25°C, fixed substrate: 4-aminobutanoate	Malus domestica
116	-	pyruvate	pH 9, 25°C, fixed substrate: 4-aminobutanoate	Malus domestica

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Release 2023.1 (January 2023)