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Literature summary for 2.6.1.19 extracted from
- Homotaurine metabolized to 3-sulfopropanoate in Cupriavidus necator H16: enzymes and genes in a patchwork pathway (2009), J. Bacteriol., 191, 6052-6058.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 4-aminobutyrate | induction during growth with 4-aminobutyrate (GABA) | Cupriavidus necator |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.3 | - |
2-oxoglutarate | Km apparent value | Cupriavidus necator | |
| 8.5 | - |
3-Aminopropanesulfonate | Km apparent value | Cupriavidus necator | |
| 9.9 | - |
4-aminobutyrate | Km apparent value | Cupriavidus necator | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
denaturated protein, determined by SDS-PAGE | Cupriavidus necator |
| 120000 | - |
mass of native protein, determined by gel filtration | Cupriavidus necator |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-aminopropanesulfonate + 2-oxoglutarate | Cupriavidus necator | substrate is homotaurine | 3-sulfopropanal + L-glutamate | - |
? | |
| 4-aminobutyrate + 2-oxoglutarate | Cupriavidus necator | - |
4-oxobutanoate + L-glutamate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cupriavidus necator | Q0K2K2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by anion exchange chromatography | Cupriavidus necator |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Cupriavidus necator | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
specific activity is 52% with oxaloacetate as amino group acceptor (normalized to that with 2-oxoglutarate (100%: 46 mkat/kg protein)), the donor is homotaurine | Cupriavidus necator |
| additional information | - |
specific activity is 59% with glyoxalate as amino group acceptor (normalized to that with 2-oxoglutarate (100%: 46 mkat/kg protein)), the donor is homotaurine | Cupriavidus necator |
| additional information | - |
specific activity is 63% with pyruvate as amino group acceptor (normalized to that with 2-oxoglutarate (100%: 46 mkat/kg protein)), the donor is homotaurine | Cupriavidus necator |
| additional information | - |
specific activity is 9% with 2-oxobutyrate as amino group acceptor (normalized to that with 2-oxoglutarate (100%: 46 mkat/kg protein)), the donor is homotaurine | Cupriavidus necator |
| additional information | - |
specific activity of 0.02 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with ammonium as the sole nitrogen source, substrate is homotaurine | Cupriavidus necator |
| additional information | - |
specific activity of 0.03 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with ammonium as the sole nitrogen source, substrate is 4-aminobutyrate | Cupriavidus necator |
| additional information | - |
specific activity of 6.5 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with 4-aminobutyrate as the sole nitrogen source, substrate is homotaurine | Cupriavidus necator |
| additional information | - |
specific activity of 8.0 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with homotaurine as the sole nitrogen source, substrate is succinate semialdehyde | Cupriavidus necator |
| additional information | - |
specific activity of 8.2 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with 4-aminobutyrate as the sole nitrogen source, substrate is 4-aminobutyrate | Cupriavidus necator |
| additional information | - |
specific activity of 8.3 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with homotaurine as the sole nitrogen source, substrate is homotaurine | Cupriavidus necator |
| additional information | - |
specific activity of 9.9 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with homotaurine as the sole nitrogen source, substrate is 4-aminobutyrate | Cupriavidus necator |
| additional information | - |
specific activity with 3-aminopropanoate, taurine, (S)-cysteate, 3-aminopropanol or 4-aminobutanol as substrates is not detected | Cupriavidus necator |
| additional information | - |
specific activity, normalized to that with homotaurine (100%: 271 mkat/kg protein), is 226% with 5-aminovalerate 6-aminocapronate as substrate | Cupriavidus necator |
| additional information | - |
specific activity, normalized to that with homotaurine (100%: 271 mkat/kg protein), is 25% with 6-aminocapronate as substrate | Cupriavidus necator |
| additional information | - |
specific activity, normalized to that with homotaurine (100%: 271 mkat/kg protein), is 270% with 4-aminobutyrate as substrate | Cupriavidus necator |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-aminopropanesulfonate + 2-oxoglutarate | substrate is homotaurine | Cupriavidus necator | 3-sulfopropanal + L-glutamate | - |
? | |
| 4-aminobutyrate + 2-oxoglutarate | - |
Cupriavidus necator | 4-oxobutanoate + L-glutamate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | gel filtration | Cupriavidus necator |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| aminobutyrate transaminase | - |
Cupriavidus necator |
| GABA transaminase | - |
Cupriavidus necator |
| GABA:2-oxoglutarate aminotransferase | - |
Cupriavidus necator |
| GabT | - |
Cupriavidus necator |
| homotaurine:2-oxoglutarate aminotransferase | - |
Cupriavidus necator |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Cupriavidus necator |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 10 | enzyme activity is detected | Cupriavidus necator |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | cofactor remains bound during purification | Cupriavidus necator | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Cupriavidus necator | transcript is detected inducibly in both homotaurine and GABA-grown cells | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | GABA metabolism | Cupriavidus necator |
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