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Literature summary for 2.6.1.19 extracted from

  • Andersen, G.; Andersen, B.; Dobritzsch, D.; Schnackerz, K.D.; Piskur, J.
    A gene duplication led to specialized gamma-aminobutyrate and beta-alanine aminotransferase in yeast (2007), FEBS J., 274, 1804-1817.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL-21(DE3) cells Lachancea kluyveri
expression as His-tagged protein Saccharomyces cerevisiae
expression as His-tagged protein Schizosaccharomyces pombe
expression as His-tagged protein Lachancea kluyveri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
2-oxoglutarate pH 8.0, 30°C Lachancea kluyveri
0.22
-
2-oxoglutarate in 100 mM potassium phosphate (pH 8.0), 0.1 mM pyridoxal 5'-phosphate Lachancea kluyveri
2.1
-
2-oxoglutarate pH 8.0, 30°C Lachancea kluyveri
3.2
-
gamma-aminobutanoate pH 8.0, 30°C Lachancea kluyveri
3.2
-
4-aminobutanoate in 100 mM potassium phosphate (pH 8.0), 0.1 mM pyridoxal 5'-phosphate Lachancea kluyveri
7.6
-
beta-Alanine pH 8.0, 30°C Lachancea kluyveri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
2 * 56000, SDS-PAGE Lachancea kluyveri
98000
-
PAGE Lachancea kluyveri
111000
-
PAGE Lachancea kluyveri

Organism

Organism UniProt Comment Textmining
Lachancea kluyveri
-
-
-
Lachancea kluyveri A5H0J5 isoform Pyd4, involved in both beta-alanine and gamma-aminobutanoic acid transamination
-
Lachancea kluyveri A5H0J6 isoform Uga1
-
Saccharomyces cerevisiae P17649 isoform Uga1
-
Schizosaccharomyces pombe O13837 isoform Uga1
-

Purification (Commentary)

Purification (Comment) Organism
nickel-chelating Sepharose chromatography and Sepharose S12 gel filtration Lachancea kluyveri

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.1
-
substrate beta-alanine, discontinuous determination of glutamate using glutamate dehydrogenase in the presence of 10 M hydrazine, pH 8.0, 30°C Lachancea kluyveri
0.13
-
substrate beta-alanine, continuous assay using malonic semialdehyde decarboxylase and alcohol dehydrogenase, pH 8.0, 30°C Lachancea kluyveri
0.14
-
substrate beta-alanine, discontinuous determination of glutamate using glutamate dehydrogenase in the presence of 10 M hydrazine, pH 8.0, 30°C Schizosaccharomyces pombe
0.15
-
substrate beta-alanine, discontinuous determination of glutamate using glutamate dehydrogenase in the presence of 10 M hydrazine, pH 8.0, 30°C Saccharomyces cerevisiae
0.75
-
substrate gamma-aminobutanoate, continuous assay with succinate dehydrogenase, pH 8.0, 30°C Lachancea kluyveri
1.6
-
substrate gamma-aminobutanoate, continuous assay using malonic semialdehyde decarboxylase and alcohol dehydrogenase, pH 8.0, 30°C Lachancea kluyveri
3.52
-
substrate beta-alanine, discontinuous determination of glutamate using glutamate dehydrogenase in the presence of 10 M hydrazine, pH 8.0, 30°C Lachancea kluyveri
3.6
-
substrate gamma-aminobutanoate, continuous assay using malonic semialdehyde decarboxylase and alcohol dehydrogenase, pH 8.0, 30°C Saccharomyces cerevisiae
3.67
-
continuous assay using malonic semialdehyde decarboxylase and alcohol dehydrogenase, pH 8.0, 30°C Schizosaccharomyces pombe
4.5
-
substrate beta-alanine, continuous assay using malonic semialdehyde decarboxylase and alcohol dehydrogenase, pH 8.0, 30°C Lachancea kluyveri
12.8
-
using 4-aminobutanoate as substrate Lachancea kluyveri
12.8
-
substrate gamma-aminobutanoate, continuous assay with succinate dehydrogenase, pH 8.0, 30°C Lachancea kluyveri

Storage Stability

Storage Stability Organism
4°C, 12 days, 57% residual activity Saccharomyces cerevisiae
4°C, 12 days, 57% residual activity Schizosaccharomyces pombe
4°C, 12 days, 57% residual activity Lachancea kluyveri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-aminobutanoate + 2-oxoglutarate
-
Lachancea kluyveri 4-oxobutanoate + L-glutamate
-
r
4-aminobutanoate + 2-oxoglutarate
-
Saccharomyces cerevisiae 4-oxobutanoate + L-glutamate
-
?
4-aminobutanoate + 2-oxoglutarate
-
Schizosaccharomyces pombe 4-oxobutanoate + L-glutamate
-
?
4-aminobutanoate + 2-oxoglutarate
-
Lachancea kluyveri 4-oxobutanoate + L-glutamate
-
?
beta-alanine + 2-oxoglutarate
-
Saccharomyces cerevisiae malonic semialdehyde + L-glutamate
-
?
beta-alanine + 2-oxoglutarate
-
Schizosaccharomyces pombe malonic semialdehyde + L-glutamate
-
?
beta-alanine + 2-oxoglutarate
-
Lachancea kluyveri malonic semialdehyde + L-glutamate
-
?
additional information does not use pyruvate as amino acceptor Lachancea kluyveri ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 56000, SDS-PAGE Lachancea kluyveri

Synonyms

Synonyms Comment Organism
gamma-aminobutyrate aminotransferase
-
Lachancea kluyveri
SkUga1p
-
Lachancea kluyveri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Lachancea kluyveri

pH Range

pH Minimum pH Maximum Comment Organism
7 9 more than 70% of maximum activity Lachancea kluyveri

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Lachancea kluyveri