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Literature summary for 2.6.1.19 extracted from
- 4-Aminobutyrate:2-oxoglutarate aminotransferase of Streptomyces griseus: purification and properties (1985), Eur. J. Biochem., 146, 101-106.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Aminooxyacetate | - |
Streptomyces griseus |  |
| carbonyl reagents | - |
Streptomyces griseus | |
| Cu2+ | - |
Streptomyces griseus | |
| D-cycloserine | - |
Streptomyces griseus | |
| D-penicillamine | - |
Streptomyces griseus | |
| gabaculine | - |
Streptomyces griseus | |
| HgCl2 | - |
Streptomyces griseus | |
| hydrazine | - |
Streptomyces griseus | |
| hydroxylamine | - |
Streptomyces griseus | |
| monoiodoacetate | - |
Streptomyces griseus | |
| additional information | no inhibition by chelating agents, non-substrate L- or D-amino acids, metal ions | Streptomyces griseus | |
| p-chloromercuribenzoate | - |
Streptomyces griseus | |
| phenylhydrazine | - |
Streptomyces griseus | |
| SH-group reagents | - |
Streptomyces griseus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.3 | - |
4-aminobutanoate | pH 8.0, 37°C | Streptomyces griseus | |
| 8.3 | - |
2-oxoglutarate | pH 8.0, 37°C | Streptomyces griseus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Streptomyces griseus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
2 * 50000, SDS-PAGE | Streptomyces griseus |
| 100000 | - |
gel filtration | Streptomyces griseus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces griseus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces griseus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.64 | - |
- |
Streptomyces griseus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-aminobutanoate + 2-oxoglutarate | best substrate | Streptomyces griseus | 4-oxobutanoate + L-glutamate | - |
r | |
| 5-aminopentanoate + 2-oxoglutarate | transamination at 60% the rate of 4-aminobutanoate | Streptomyces griseus | L-glutamate + 5-oxopentanoate | - |
? | |
| 6-aminohexanoate + 2-oxoglutarate | transamination at 29% the rate of 4-aminobutanoate | Streptomyces griseus | L-glutamate + 6-oxohexanoate | - |
? | |
| D-lysine + 2-oxoglutarate | poor amino donor | Streptomyces griseus | L-glutamate + 5-aminopentanal | - |
r | |
| DL-ornithine + 2-oxoglutarate | poor amino donor | Streptomyces griseus | L-glutamate + 4-methyl-2-oxopentanoate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 50000, SDS-PAGE | Streptomyces griseus |
| More | amino acid composition | Streptomyces griseus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Streptomyces griseus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | linear increase, above 55°C rapid decrease | Streptomyces griseus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
pyridoxal phosphate protects against heat inactivation | Streptomyces griseus |
| 45 | - |
up to, at least 10 min stable, pH 8 | Streptomyces griseus |
| 50 | - |
above, 10 min, inactivation | Streptomyces griseus |
| 50 | - |
up to, stable | Streptomyces griseus |
| 60 | - |
10 min stable in the presence of pyridoxal phosphate | Streptomyces griseus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | - |
Streptomyces griseus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
no activity below | Streptomyces griseus |
| 11 | - |
and above, no activity | Streptomyces griseus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | 15 min stable, 37°C | Streptomyces griseus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | 2 mol per mol enzyme | Streptomyces griseus | |
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