Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | - |
Rattus norvegicus | |
Succinic semialdehyde | substrate inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic study | Rattus norvegicus | |
additional information | - |
additional information | pH-dependence of kinetic data | Rattus norvegicus | |
4 | - |
4-aminobutanoate | 38°C, pH 8.5 | Rattus norvegicus | |
5.5 | - |
2-oxoglutarate | 38°C, pH 8.5 | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
2 * 57000, SDS-PAGE with or without DTT or urea | Rattus norvegicus |
103000 | - |
PAGE | Rattus norvegicus |
105000 | - |
gel filtration | Rattus norvegicus |
107000 | - |
calculated from amino acid composition | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10 | - |
38°C, pH 8.5 | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminobutanoate + 2-oxoglutarate | - |
Rattus norvegicus | 4-oxobutanoate + L-glutamate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 57000, SDS-PAGE with or without DTT or urea | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Rattus norvegicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 55 | about half-maximal activity at 30°C and 55°C | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
t1/2: 3 h | Rattus norvegicus |
50 | - |
3 h, 80% loss of activity | Rattus norvegicus |
55 | - |
3 h, inactivation | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 6.8 | Rattus norvegicus |
8.5 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.4 | 9 | about half-maximal activity at pH 7.4 and about 90% of maximal activity at pH 9 | Rattus norvegicus |
8 | 9.2 | about 50% of maximal activity at pH 9.2 | Rattus norvegicus |
8 | 9.2 | about half-maximal activity at pH 8 | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00018 | - |
2-oxoglutarate | 38°C, pH 8.5 | Rattus norvegicus | |
0.0066 | - |
Succinic semialdehyde | 38°C, pH 8.5 | Rattus norvegicus |