Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sphaerobacter thermophilus |
Organic Solvent | Comment | Organism |
---|---|---|
DMSO | 20%, 43% residual activity after 12 h | Sphaerobacter thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphaerobacter thermophilus | D1C218 | - |
- |
Sphaerobacter thermophilus DSM 20745 | D1C218 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.3 | - |
substrate rac-beta-phenylalanine, 37°C, pH 8.0 | Sphaerobacter thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-amino-3-(3,4-dimethoxyphenyl)propanoate + pyruvate | about 95% of the amine donor activity of 4-amino-4-(4-methylphenyl)butanoic acid | Sphaerobacter thermophilus | 3-oxo-3-(3,4-dimethoxyphenyl)propanoate + L-alanine | - |
? | |
3-amino-3-(3,4-dimethoxyphenyl)propanoate + pyruvate | about 95% of the amine donor activity of 4-amino-4-(4-methylphenyl)butanoic acid | Sphaerobacter thermophilus DSM 20745 | 3-oxo-3-(3,4-dimethoxyphenyl)propanoate + L-alanine | - |
? | |
3-amino-3-(4-fluorophenyl)propanoate + pyruvate | about 80% of the amine donor activity of 4-amino-4-(4-methylphenyl)butanoic acid | Sphaerobacter thermophilus | 3-oxo-3-(4-fluorophenyl)propanoate + L-alanine | - |
? | |
3-amino-3-(4-fluorophenyl)propanoate + pyruvate | about 80% of the amine donor activity of 4-amino-4-(4-methylphenyl)butanoic acid | Sphaerobacter thermophilus DSM 20745 | 3-oxo-3-(4-fluorophenyl)propanoate + L-alanine | - |
? | |
3-amino-5-methylhexanoate + 2-oxohexanoate | - |
Sphaerobacter thermophilus | 3-oxo-5-methylhexanoate + 2-aminohexanoate | about 65% of the amine acceptor activity of pyruvate | ? | |
3-amino-5-methylhexanoate + 2-oxohexanoate | - |
Sphaerobacter thermophilus DSM 20745 | 3-oxo-5-methylhexanoate + 2-aminohexanoate | about 65% of the amine acceptor activity of pyruvate | ? | |
3-amino-5-methylhexanoate + ethyl 2-oxopropanoate | - |
Sphaerobacter thermophilus | 3-oxo-5-methylhexanoate + ethyl 2-aminopropanoate | about 70% of the amine acceptor activity of pyruvate | ? | |
3-amino-5-methylhexanoate + ethyl 2-oxopropanoate | - |
Sphaerobacter thermophilus DSM 20745 | 3-oxo-5-methylhexanoate + ethyl 2-aminopropanoate | about 70% of the amine acceptor activity of pyruvate | ? | |
3-amino-5-methylhexanoate + pyruvate | about 85% of the amine donor activity of 4-amino-4-(4-methylphenyl)butanoic acid | Sphaerobacter thermophilus | 3-oxo-5-methylhexanoate + L-alanine | - |
? | |
4-amino-4-(4-methylphenyl)butanoic acid + pyruvate | - |
Sphaerobacter thermophilus | 4-oxo-4-(4-methylphenyl)butanoate + L-alanine | - |
? | |
additional information | enzyme has activity towards abroad range of substrates including amines, beta- and gamma-amino acids | Sphaerobacter thermophilus | ? | - |
? | |
additional information | enzyme has activity towards abroad range of substrates including amines, beta- and gamma-amino acids | Sphaerobacter thermophilus DSM 20745 | ? | - |
? | |
rac-beta-phenylalanine + pyruvate | - |
Sphaerobacter thermophilus | 3-oxo-3-phenylpropanoate + L-alanine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Sthe_0848 | - |
Sphaerobacter thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
stable for at least 1 h | Sphaerobacter thermophilus |