Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21 Star (DE3) pLysS | Chromobacterium violaceum |
Crystallization (Comment) | Organism |
---|---|
microbatch method using either 0.05 M HEPES pH 7.5, 5% (v/v) 2-propanol, 10%(w/v) PEG 4000 or 0.1 M lithium sulfate monohydrate, 0.05 M Tris-HCl pH 8.5, 15% (w/v) PEG 4000 | Chromobacterium violaceum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
SDS-PAGE | Chromobacterium violaceum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chromobacterium violaceum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel Sepharose Hi-Performance column chromatography and Superdex 200 Hiload 16/60 gel filtration | Chromobacterium violaceum |
Synonyms | Comment | Organism |
---|---|---|
omega-amino acid:pyruvate transaminase | - |
Chromobacterium violaceum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Chromobacterium violaceum |