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Literature summary for 2.6.1.16 extracted from

  • Sanchez-Lopez, J.F.; Gonzalez-Ibarra, J.; Alvarez-Vargas, A.; Milewski, S.; Villagomez-Castro, J.C.; Cano-Canchola, C.; Lopez-Romero, E.
    Isolation of the GFA1 gene encoding glucosamine-6-phosphate synthase of Sporothrix schenckii and its expression in Saccharomyces cerevisiae (2015), Protein Expr. Purif., 110, 57-64.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Sporothrix schenckii

Protein Variants

Protein Variants Comment Organism
biotechnology the recombinant enzyme expressed in Saccharomyces cereviaise reveals some differences from the wild type enzyme, such as improved stability and less sensitivity to UDP-GlcNAc Sporothrix schenckii

Inhibitors

Inhibitors Comment Organism Structure
2-amino-2-deoxy-D-mannitol 6-phosphate
-
Sporothrix schenckii
N3-(4-Methoxyfumaroyl)-L-2,3-diaminopropanoic acid selective Sporothrix schenckii
UDP-N-acetylglucosamine maximum inhibition at 1 mM Sporothrix schenckii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
79000
-
x * 79000, SDS-PAGE Sporothrix schenckii

Organism

Organism UniProt Comment Textmining
Sporothrix schenckii D3YIG6
-
-

Storage Stability

Storage Stability Organism
-20°C, potassium phosphate buffer, pH 7.0, containing 1 mM EDTA, 1 mM DTT, 50% glycerol and 10 mM fructose 6-phosphate, fully stable Sporothrix schenckii
4°C, potassium phosphate buffer, pH 7.0, containing 1 mM EDTA, 1 mM DTT, 50% glycerol and 10 mM fructose 6-phosphate, complete loss of activity within 24 h Sporothrix schenckii

Subunits

Subunits Comment Organism
? x * 79000, SDS-PAGE Sporothrix schenckii

Synonyms

Synonyms Comment Organism
Gfa1
-
Sporothrix schenckii

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.013
-
pH not specified in the publication, temperature not specified in the publication Sporothrix schenckii N3-(4-Methoxyfumaroyl)-L-2,3-diaminopropanoic acid
0.073
-
pH not specified in the publication, temperature not specified in the publication Sporothrix schenckii 2-amino-2-deoxy-D-mannitol 6-phosphate
0.45
-
crude enzyme preparation, pH not specified in the publication, temperature not specified in the publication Sporothrix schenckii UDP-N-acetylglucosamine

General Information

General Information Comment Organism
physiological function the recombinant enzyme restores glucosamine prototrophy of the Saccharomyces cerevisiae gfa1 null mutant Sporothrix schenckii