Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
molecular model of the 3-D on the basis of the crystal structures of Escherichia coli GlcN-6-P synthase, PDB entry 4AMV and Candida albicans GlcN-6-P synthase, PDB entry 2POC. The protein has a terminal glutamine amidotransferase type 2 domain (residues 2-318). The phosphosugar-binding SIS domain (residues 390-529; 562-707) plays a key role in catalysis and communication of fructose 6-phosphate and D-glucosamine 6-phosphate between the two active sites | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
D-fructose 6-phosphate | pH 7.0, 37°C | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
- |
Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P14742 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.93 | - |
pH 7.0, 37°C | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + D-fructose 6-phosphate | - |
Saccharomyces cerevisiae | L-glutamate + D-glucosamine 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 80047, calculated, x * 90000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
Gfa1 | - |
Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 60 | - |
Saccharomyces cerevisiae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 5.5 | - |
Saccharomyces cerevisiae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Saccharomyces cerevisiae | calculated | - |
6 |